Detection of pyridinium cross-links in human bile

This study evaluated whether pyridinium cross-links, which are positively charged, besides renal clearance are also cleared by the liver into bile. In 13 human bile samples tested, we were able to detect both pyridinoline (PYD) and deoxypyridinoline (DPD) in small amounts which were estimated to be...

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Bibliographic Details
Published in:Calcified tissue international 1995-12, Vol.57 (6), p.415-418
Main Authors: Romanello, M, Noris Suarez, K, Bettica, P, Moro, L
Format: Article
Language:English
Subjects:
Adult
Aged
Amino Acids - metabolism
Bile - metabolism
Cross-Linking Reagents
Female
Humans
Hydroxylysine - analogs & derivatives
Hydroxylysine - metabolism
Male
Middle Aged
Pyridinium Compounds - chemistry
Pyridinium Compounds - metabolism
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Summary:This study evaluated whether pyridinium cross-links, which are positively charged, besides renal clearance are also cleared by the liver into bile. In 13 human bile samples tested, we were able to detect both pyridinoline (PYD) and deoxypyridinoline (DPD) in small amounts which were estimated to be about 1-2% of the amount usually found in urine. To further evaluate the amount of pyridinium cross-links excreted through bile, we studied the stability of these compounds at the alkaline pH of bile. No effect on their stability was detected over a 6-hour incubation. The origin of these molecules in bile and the significance of this finding in the use of PYD and DPD as bone resorption markers are discussed.
ISSN:0171-967X
1432-0827
DOI:10.1007/BF00301942