Blocking of HIV-1 Infectivity by a Soluble, Secreted Form of the CD4 Antigen

The initial event in the infection of human T lymphocytes, macrophages, and other cells by human immunodeficiency virus (HIV-1) is the attachment of the HIV-1 envelope glycoprotein gp120 to its cellular receptor, CD4. As a step toward designing antagonists of this binding event, soluble, secreted fo...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-12, Vol.238 (4834), p.1704-1707
Main Authors: Smith, Douglas H., Byrn, Randal A., Marsters, Scot A., Gregory, Timothy, Groopman, Jerome E., Capon, Daniel J.
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
Acquired Immunodeficiency Syndrome - immunology
AIDS
AIDS/HIV
Animals
Antigen presenting cells
Antigens
Antigens, Differentiation, T-Lymphocyte - immunology
Antiviral drugs
Biological and medical sciences
Biotechnology
Cell Line
CHO cells
Cultured cells
Fundamental and applied biological sciences. Psychology
Giant cells
Glycoproteins
Health care
Health. Pharmaceutical industry
HIV
HIV (Viruses)
HIV - immunology
HIV - pathogenicity
HIV - physiology
HIV 1
Human immunodeficiency virus 1
Humans
Immunology, Experimental
Industrial applications and implications. Economical aspects
Infections
Microbiology
Production of active biomolecules
Protein research
Receptors, Virus - immunology
Receptors, Virus - physiology
Recombinant Proteins - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
T lymphocytes
T-Lymphocytes - immunology
Viral Envelope Proteins - immunology
Viral Envelope Proteins - physiology
Virology
Viruses
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Summary:The initial event in the infection of human T lymphocytes, macrophages, and other cells by human immunodeficiency virus (HIV-1) is the attachment of the HIV-1 envelope glycoprotein gp120 to its cellular receptor, CD4. As a step toward designing antagonists of this binding event, soluble, secreted forms of CD4 were produced by transfection of mammalian cells with vectors encoding versions of CD4 lacking its transmembrane and cytoplasmic domains. The soluble CD4 so produced binds gp120 with an affinity and specificity comparable to intact CD4 and is capable of neutralizing the infectivity of HIV-1. These studies reveal that the high-affinity CD4-gp120 interaction does not require other cell or viral components and may establish a novel basis for therapeutic intervention in the acquired immune deficiency syndrome (AIDS).
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3500514