Role of Iron Chelators in Growth-Promoting Effect on Mouse Hybridoma Cells in a Chemically Defined Medium

The role of various iron chelators on the multiplication of mouse hybridoma cells in an albumin-free, transferrin-deficient defined medium was investigated. Fe(III)-dihydroxyethylglycine, Fe(III)-glycylglycine, Fe(III)-ethylenediamine-N,N'-dipropionic acid, or Fe(III)-iminodiacetic acid support...

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Published in:In Vitro Cellular & Developmental Biology 1987-12, Vol.23 (12), p.815-820
Main Authors: Noritsugu Yabe, Miwa Kato, Matsuya, Yutaka, Isao Yamane, Muneaki Iizuka, Hiroyuki Takayoshi, Kiyokazu Suzuki
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Biotechnology
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell Division - drug effects
Cell growth
Chlorides
Culture Media - pharmacology
Cultured cells
Dose-Response Relationship, Drug
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Ferric Compounds - pharmacology
Free radicals
Fundamental and applied biological sciences. Psychology
Growth Substances - pharmacology
Hybridomas
Hybridomas - cytology
Hybridomas - drug effects
Imino acids
Iron
Iron Chelating Agents - pharmacology
Iron deficiency anemia
Methods. Procedures. Technologies
Mice
Mice, Inbred BALB C
Molecular and cellular biology
Serum Albumin
Time Factors
Transferrin - pharmacology
Transferrins
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Summary:The role of various iron chelators on the multiplication of mouse hybridoma cells in an albumin-free, transferrin-deficient defined medium was investigated. Fe(III)-dihydroxyethylglycine, Fe(III)-glycylglycine, Fe(III)-ethylenediamine-N,N'-dipropionic acid, or Fe(III)-iminodiacetic acid supported the excellent growth of the cells. In addition, the growth of the iron-starved cells, which had been preincubated in a protein-, iron- and chelator-free defined medium, restored rapidly when the medium was supplemented with holotransferrin, ferric iron, and chelator compared to that when supplemented with holotransferrin, but without iron and chelator. The results suggest that such chelators modulate a progression of transferrin cycle in the presence of transferrin and ferric iron. An alternative explanation is that there is a decrease in generation of iron-catalyzed free radicals.
ISSN:0883-8364
2327-431X
1475-2689
DOI:10.1007/BF02620959