Role of Iron Chelators in Growth-Promoting Effect on Mouse Hybridoma Cells in a Chemically Defined Medium

• Published in: In Vitro Cellular & Developmental Biology 1987-12, Vol.23 (12), p.815-820
• Main Authors: Noritsugu Yabe, Miwa Kato, Matsuya, Yutaka, Isao Yamane, Muneaki Iizuka, Hiroyuki Takayoshi, Kiyokazu Suzuki
• Format: Article
• Language: English
• Subjects: Animal cells; Animals; Biological and medical sciences; Biotechnology; Cell culture techniques; Cell cultures. Hybridization. Fusion; Cell Division - drug effects; Cell growth; Chlorides; Culture Media - pharmacology; Cultured cells; Dose-Response Relationship, Drug; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Ferric Compounds - pharmacology; Free radicals; Fundamental and applied biological sciences. Psychology; Growth Substances - pharmacology; Hybridomas; Hybridomas - cytology; Hybridomas - drug effects; Imino acids; Iron; Iron Chelating Agents - pharmacology; Iron deficiency anemia; Methods. Procedures. Technologies; Mice; Mice, Inbred BALB C; Molecular and cellular biology; Serum Albumin; Time Factors; Transferrin - pharmacology; Transferrins
• ISSN: 0883-8364; 2327-431X; 1475-2689
• DOI: 10.1007/BF02620959

The role of various iron chelators on the multiplication of mouse hybridoma cells in an albumin-free, transferrin-deficient defined medium was investigated. Fe(III)-dihydroxyethylglycine, Fe(III)-glycylglycine, Fe(III)-ethylenediamine-N,N'-dipropionic acid, or Fe(III)-iminodiacetic acid supported the excellent growth of the cells. In addition, the growth of the iron-starved cells, which had been preincubated in a protein-, iron- and chelator-free defined medium, restored rapidly when the medium was supplemented with holotransferrin, ferric iron, and chelator compared to that when supplemented with holotransferrin, but without iron and chelator. The results suggest that such chelators modulate a progression of transferrin cycle in the presence of transferrin and ferric iron. An alternative explanation is that there is a decrease in generation of iron-catalyzed free radicals.