Use of a monoclonal antibody to ovine IgE for fly strike studies in sheep

A new monoclonal raised against sheep IgE was used to examine sera and wound exudates from sheep which had been struck by Lucilia cuprina in the field. The antibody was also used to detect the presence of IgE in sera and skin sections from sheep which had been artificially infected with fly larvae 3...

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Bibliographic Details
Published in:International journal for parasitology 1995-12, Vol.25 (12), p.1505-1507
Main Authors: MacDiarmid, J.A., Clarke, R., McClure, S.J., Bowen, F.R., Burrell, D.H.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antibody Specificity
Biological and medical sciences
Calliphoridae
Diptera
Diptera - immunology
Immunoglobulin E - blood
Immunoglobulin E - metabolism
Infectious diseases
Lucilia cuprina
Medical sciences
Miscellaneous
Myiasis - immunology
Myiasis - veterinary
Parasitic diseases
Sheep
Sheep Diseases - immunology
Skin - immunology
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Summary:A new monoclonal raised against sheep IgE was used to examine sera and wound exudates from sheep which had been struck by Lucilia cuprina in the field. The antibody was also used to detect the presence of IgE in sera and skin sections from sheep which had been artificially infected with fly larvae 3 times. Neither total, nor L. cuprina specific circulating IgE could be detected in serum or wound exudates from struck sheep. Cell bound IgE was, however, identified by the monoclonal in skin sections from struck sheep and from a control sheep which had not been struck. No difference in the number of IgE positive cells was observed between the control and 2 of the 3 artificially infected sheep, and none of the latter showed an increase in IgE positive cells even after 3 infections. One sheep showed twice as many IgE positive cells as the other treated sheep and the third larval infection was difficult to establish and limited in size and severity. This suggests a relationship between innate resistance to strike and the number of IgE positive cells present in skin.
ISSN:0020-7519
1879-0135
DOI:10.1016/0020-7519(95)00027-5