Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels

Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I...

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Published in:Clinical and diagnostic virology 1995-08, Vol.4 (2), p.149-161
Main Authors: Fujiyama, Chihaya, Fujiyoshi, Toshinobu, Matsumoto, Daiten, Yashiki, Shinji, Tamashiro, Hidehiko, Sonoda, Shunro
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HTLV-I
HTLV-II
Western blot criterion
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container_issue 2
container_start_page 149
container_title Clinical and diagnostic virology
container_volume 4
creator Fujiyama, Chihaya
Fujiyoshi, Toshinobu
Matsumoto, Daiten
Yashiki, Shinji
Tamashiro, Hidehiko
Sonoda, Shunro
description Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Study design: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel which had been established in our previous study. Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (⩾0.980) for p28, p53 and gp46, but low R-values (
doi_str_mv 10.1016/0928-0197(95)00008-V
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There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Study design: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel which had been established in our previous study. Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (⩾0.980) for p28, p53 and gp46, but low R-values (&lt;0.900) for p19 and p24. Eitest ATL-WB showed a high R-value for p19, but low R-values for p53, gp46 and rgp21. HTLV BLOT 2.2 showed high R-values for p28 and recombinant gp46-I, but low R-values for p53 and gp46. The HTLV-II serum panel showed positive reactions with p24 and p19 of HTLV-I but a little if any reactions with p28 and gp46 of HTLV-I. Although these reactions could not define HTLV-II-specific WB patterns in anti-HTLV-I WB, HTLV-I- and HTLV-II-specific recombinant gp46 in HTLV BLOT 2.3 were useful to distinguish anti-HTLV-II antibodies. Conclusions: The currently available anti-HTLV-I WB kits are sufficient for confirmatory testing of anti-HTLV-I antibodies with WHO criteria using one gag and one env positivity, but they are inadequate for anti-HTLV-II confirmatory testing.</description><identifier>ISSN: 0928-0197</identifier><identifier>EISSN: 1873-4901</identifier><identifier>DOI: 10.1016/0928-0197(95)00008-V</identifier><identifier>PMID: 15566836</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>HTLV-I ; HTLV-II ; Western blot criterion</subject><ispartof>Clinical and diagnostic virology, 1995-08, Vol.4 (2), p.149-161</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c273t-97fa8854e47ec4e9a03ae4957572709f64c7033f04e94e2b4b811f4cf0db3a433</citedby><cites>FETCH-LOGICAL-c273t-97fa8854e47ec4e9a03ae4957572709f64c7033f04e94e2b4b811f4cf0db3a433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15566836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujiyama, Chihaya</creatorcontrib><creatorcontrib>Fujiyoshi, Toshinobu</creatorcontrib><creatorcontrib>Matsumoto, Daiten</creatorcontrib><creatorcontrib>Yashiki, Shinji</creatorcontrib><creatorcontrib>Tamashiro, Hidehiko</creatorcontrib><creatorcontrib>Sonoda, Shunro</creatorcontrib><title>Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels</title><title>Clinical and diagnostic virology</title><addtitle>Clin Diagn Virol</addtitle><description>Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Study design: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel which had been established in our previous study. Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (⩾0.980) for p28, p53 and gp46, but low R-values (&lt;0.900) for p19 and p24. Eitest ATL-WB showed a high R-value for p19, but low R-values for p53, gp46 and rgp21. HTLV BLOT 2.2 showed high R-values for p28 and recombinant gp46-I, but low R-values for p53 and gp46. The HTLV-II serum panel showed positive reactions with p24 and p19 of HTLV-I but a little if any reactions with p28 and gp46 of HTLV-I. Although these reactions could not define HTLV-II-specific WB patterns in anti-HTLV-I WB, HTLV-I- and HTLV-II-specific recombinant gp46 in HTLV BLOT 2.3 were useful to distinguish anti-HTLV-II antibodies. Conclusions: The currently available anti-HTLV-I WB kits are sufficient for confirmatory testing of anti-HTLV-I antibodies with WHO criteria using one gag and one env positivity, but they are inadequate for anti-HTLV-II confirmatory testing.</description><subject>HTLV-I</subject><subject>HTLV-II</subject><subject>Western blot criterion</subject><issn>0928-0197</issn><issn>1873-4901</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMoun78A5GcRA_RpEma5iKI-LGwIMjuegxpOpVIt12TVvDfm3WL3swlgXnemcmD0CmjV4yy_JrqrCCUaXWh5SVNpyDLHTRhheJEaMp20eQXOUCHMb7TFNNC7qMDJmWeFzyfoMULEPi0zWB737W4q7Fte0-e5rMlmeJXiD2EFpdN12Mbo_3CQ_TtGx7rtq3G5xRHCMMKr20LTTxGe7VtIpyM9xFaPNzP757I7Plxenc7Iy5TvCda1bYopAChwAnQlnILQkslVaaornPhFOW8pqkmICtFWTBWC1fTquRWcH6Ezrd916H7GNKyZuWjg6ZJW3RDNEppmWU5S6DYgi50MQaozTr4lQ1fhlGz0Wk2rszGldHS_Og0yxQ7G_sP5Qqqv9DoLwE3WyB9Gj49BBOdh9ZB5QO43lSd_3_CN9x3gf4</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Fujiyama, Chihaya</creator><creator>Fujiyoshi, Toshinobu</creator><creator>Matsumoto, Daiten</creator><creator>Yashiki, Shinji</creator><creator>Tamashiro, Hidehiko</creator><creator>Sonoda, Shunro</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199508</creationdate><title>Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels</title><author>Fujiyama, Chihaya ; Fujiyoshi, Toshinobu ; Matsumoto, Daiten ; Yashiki, Shinji ; Tamashiro, Hidehiko ; Sonoda, Shunro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c273t-97fa8854e47ec4e9a03ae4957572709f64c7033f04e94e2b4b811f4cf0db3a433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>HTLV-I</topic><topic>HTLV-II</topic><topic>Western blot criterion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiyama, Chihaya</creatorcontrib><creatorcontrib>Fujiyoshi, Toshinobu</creatorcontrib><creatorcontrib>Matsumoto, Daiten</creatorcontrib><creatorcontrib>Yashiki, Shinji</creatorcontrib><creatorcontrib>Tamashiro, Hidehiko</creatorcontrib><creatorcontrib>Sonoda, Shunro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and diagnostic virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujiyama, Chihaya</au><au>Fujiyoshi, Toshinobu</au><au>Matsumoto, Daiten</au><au>Yashiki, Shinji</au><au>Tamashiro, Hidehiko</au><au>Sonoda, Shunro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels</atitle><jtitle>Clinical and diagnostic virology</jtitle><addtitle>Clin Diagn Virol</addtitle><date>1995-08</date><risdate>1995</risdate><volume>4</volume><issue>2</issue><spage>149</spage><epage>161</epage><pages>149-161</pages><issn>0928-0197</issn><eissn>1873-4901</eissn><abstract>Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Study design: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel which had been established in our previous study. Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (⩾0.980) for p28, p53 and gp46, but low R-values (&lt;0.900) for p19 and p24. Eitest ATL-WB showed a high R-value for p19, but low R-values for p53, gp46 and rgp21. HTLV BLOT 2.2 showed high R-values for p28 and recombinant gp46-I, but low R-values for p53 and gp46. The HTLV-II serum panel showed positive reactions with p24 and p19 of HTLV-I but a little if any reactions with p28 and gp46 of HTLV-I. Although these reactions could not define HTLV-II-specific WB patterns in anti-HTLV-I WB, HTLV-I- and HTLV-II-specific recombinant gp46 in HTLV BLOT 2.3 were useful to distinguish anti-HTLV-II antibodies. Conclusions: The currently available anti-HTLV-I WB kits are sufficient for confirmatory testing of anti-HTLV-I antibodies with WHO criteria using one gag and one env positivity, but they are inadequate for anti-HTLV-II confirmatory testing.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15566836</pmid><doi>10.1016/0928-0197(95)00008-V</doi><tpages>13</tpages></addata></record>
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subjects HTLV-I
HTLV-II
Western blot criterion
title Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels
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