Is enzyme release a sign of irreversible injury of cardiomyocytes?

The amount of creatine kinase (CK) release (percent of releasable CK) and the amount of irreversibly injured cardiomyocytes evaluated by counting trypan blue stained nuclei (percent of total) was investigated in isolated perfused rat hearts under various conditions: Intermittent contractile depressi...

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Bibliographic Details
Published in:Life sciences (1973) 1996, Vol.58 (7), p.545-550
Main Authors: Hüser, Monika, Stegemann, Emilia, Kammermeier, Helmut
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
cell death
Cell Hypoxia
Creatine Kinase - metabolism
enzyme release
hypoxia
Myocardium - enzymology
Myocardium - metabolism
Myocardium - pathology
Oxygen - metabolism
Rats
reoxygenation
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Summary:The amount of creatine kinase (CK) release (percent of releasable CK) and the amount of irreversibly injured cardiomyocytes evaluated by counting trypan blue stained nuclei (percent of total) was investigated in isolated perfused rat hearts under various conditions: Intermittent contractile depression by low calcium (0.5 mM) and by administration of BDM (10 mM) as well as by anoxia / reoxygenation. For comparison severe injury induced by calcium paradox was also studied. CK release amounted to 0.5 % to 3 % (controls 15 to 105 min) and to 3 to 5 % for the interventions and about 40 % for calcium paradox. Irreversibly injured myocytes amounted to 0.1 to 0.3 % and 0.3 to 0.5 % respectively and to about 40 % in calcium paradox. Thus, the percentage of enzyme release exceeded the percentage of irreversibly injured cells by more than one order of magnitude under all experimental conditions, including controls, except for calcium paradox where the percentages were the same. We conclude that cytosolic enzymes can be released to substantial amounts without irreversible injury of cardiomyocytes under various conditions, and only with severe membrane lesions (Ca paradox) enzyme release reflects irreversible injury.
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(95)02262-7