Immunohistochemical detection of rinderpest virus: effects of autolysis and period of fixation

Samples of eyelid, tongue, soft palate and palatine tonsil were collected from calves infected experimentally with rinderpest virus. The tissues were fixed in 10 per cent neutral buffered formalin immediately, 24 or 48 hours post mortem. Then, after three days, 10 days, 28 days or three months in fo...

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Bibliographic Details
Published in:Research in veterinary science 1996-03, Vol.60 (2), p.182-184
Main Authors: Brown, C.C, Ojok, L, Mariner, J.C
Format: Article
Language:English
Subjects:
Animals
Autolysis
Cattle
Eyelids - pathology
Eyelids - virology
Formaldehyde
Histological Techniques
Immunohistochemistry - methods
Palate, Soft - pathology
Palate, Soft - virology
Palatine Tonsil - pathology
Palatine Tonsil - virology
Postmortem Changes
Rinderpest - diagnosis
Rinderpest - pathology
Rinderpest - virology
rinderpest virus
Rinderpest virus - isolation & purification
Time Factors
Tongue - pathology
Tongue - virology
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Summary:Samples of eyelid, tongue, soft palate and palatine tonsil were collected from calves infected experimentally with rinderpest virus. The tissues were fixed in 10 per cent neutral buffered formalin immediately, 24 or 48 hours post mortem. Then, after three days, 10 days, 28 days or three months in formalin, they were processed into paraffin blocks and examined innnunohistochemically for rinderpest viral antigen. The tonsil was the best of the four tissues in providing a consistently positive immunohistochemical signal for the presence of virus, despite autolytic changes and/or prolonged fixation.
ISSN:0034-5288
1532-2661
DOI:10.1016/S0034-5288(96)90016-8