Degradation of poly( d,l-lactic acid) nanoparticles coated with albumin in model digestive fluids (USP XXII)

Entirely biodegradable poly( d,l-lactic acid) (PLA 50) nanoparticles coated with albumin were prepared by the solvent evaporation technique. Their degradative properties were investigated in simulated gastric and intestinal fluids (USP XXII). The degradation of the albumin coating was monitored by H...

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Bibliographic Details
Published in:Biomaterials 1996-04, Vol.17 (7), p.715-723
Main Authors: Landry, F.B., Bazile, D.V., Spenlehauer, G., Veillard, M., Kreuter, J.
Format: Article
Language:English
Subjects:
albumin
Albumins - metabolism
Animals
Biological and medical sciences
Body Fluids - metabolism
Chromatography
Chromatography, High Pressure Liquid
Delayed-Action Preparations
Drug Delivery Systems
enzymatic degradation
Freeze Fracturing
gastric fluid
Gastric Juice - metabolism
Humans
Hydrogen-Ion Concentration
intestinal fluid
Lactates - analysis
Lactates - metabolism
Lactic Acid
Medical sciences
Microscopy, Electron
Pancreas - enzymology
Pancreatin - metabolism
Poly( d,l-lactic acid)
Polyesters
Polymers - metabolism
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Swine
Technology. Biomaterials. Equipments. Material. Instrumentation
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Summary:Entirely biodegradable poly( d,l-lactic acid) (PLA 50) nanoparticles coated with albumin were prepared by the solvent evaporation technique. Their degradative properties were investigated in simulated gastric and intestinal fluids (USP XXII). The degradation of the albumin coating was monitored by HPLC, whereas PLA 50 degradation was determined by size exclusion chromatography (SEC) as well as by the detection of lactate in bulk solution by enzymatic assay. As expected, the coating effect of albumin, a readily digestible protein, rapidly disappeared in both gastric and intestinal media, thus exposing albumin-free PLA 50 cores to hydrolytic processes. In pepsin-rich simulated gastric fluid, no degradation of the PLA 50 core was observed over 8 h incubation time. In contrast, in pancreatin-rich simulated intestinal fluid, the PLA 50 nanoparticles were rapidly converted into lactate. The results showed that the PLA 50 degradation was mainly due to an enzymatic cleavage process. Further experiments showed the involvement of lipases in the degradation of the PLA 50 core in simulated intestinal fluid.
ISSN:0142-9612
1878-5905
DOI:10.1016/0142-9612(96)86742-1