Urinary and Biliary Metabolites of Genistein in Rats

Examination was made of the urinary and biliary excretion of the metabolites of genistein and genistin, the major components of Glycine and Sophora genus in rats. The urine of rats administered genistein orally contained eight metabolites. Three of these metabolites, genistein 4'-O-sulfate (M-1...

Full description

Saved in:
Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1996/03/15, Vol.19(3), pp.413-417
Main Authors: YASUDA, Takaaki, MIZUNUMA, Shuichi, KANO, Yoshihiro, SAITO, Kenichi, OHSAWA, Keisuke
Format: Article
Language:English
Subjects:
Animals
Bile - metabolism
biliary excretion
Biological and medical sciences
Biotransformation
Chromatography, Gas
Chromatography, High Pressure Liquid
Enzyme Inhibitors - pharmacokinetics
Enzyme Inhibitors - urine
Genistein
genistin
Hydrolysis
Isoflavones - pharmacokinetics
Isoflavones - urine
Magnetic Resonance Spectroscopy
Male
Medical sciences
metabolite
Miscellaneous
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
Sulfates - analysis
urinary excretion
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Examination was made of the urinary and biliary excretion of the metabolites of genistein and genistin, the major components of Glycine and Sophora genus in rats. The urine of rats administered genistein orally contained eight metabolites. Three of these metabolites, genistein 4'-O-sulfate (M-1), genistein 7-O-β-D-glucuronide (M-3), genistein 4'-O-sulfate 7-O-β-D-glucuronide (M-6), were identified from spectroscopic and chemical data. The bile of rats administered genistein orally contained M-2, M-3 and M-6. M-6, a major biliary metabolite, was isolated and identified from spectroscopic and chemical data. The urine or bile of rats treated with genistin, the glycoside of genistein, contained M-1-M-8 or M-2, M-3, M-6 in the above metabolites. These findings suggest that genistin is absorbed as genistein after hydrolysis in the gastrointestinal tract. The total cumulative amounts of the two metabolites and genistein excreted in the urine during 48h, or of M-6 excreted in the bile during 36h following the oral administration of genistein, were approximately 5.7% or 16.0% of the doses administered, respectively. The results show that M-1, M-3 and M-6, having a free hydroxyl, glucuronide-or sulfate-conjugated hydroxyls at the C-7 or C-4' position, are excreted in the urine and bile as parts of the metabolites of genistein.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.19.413