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Novel anthocyanins produced in petals of genetically transformed lisianthus
The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose: flavonoid-3- O-glucosyltransferase cDNA from Antirrhinum majus, have been studied using paper chromatography, HPLC and 1H and 13C NMR spectroscopy involving the use of 1H 1H COSY and TOCS...
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| Published in: | Phytochemistry (Oxford) 1996-07, Vol.42 (4), p.1035-1038 |
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| Main Author: | |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c386t-928021eaba33ffa41901d44f370fd15b31fa31daa849b4a803c093caa4ced2c03 |
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| cites | cdi_FETCH-LOGICAL-c386t-928021eaba33ffa41901d44f370fd15b31fa31daa849b4a803c093caa4ced2c03 |
| container_end_page | 1038 |
| container_issue | 4 |
| container_start_page | 1035 |
| container_title | Phytochemistry (Oxford) |
| container_volume | 42 |
| creator | Markham, Kenneth R. |
| description | The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose: flavonoid-3-
O-glucosyltransferase cDNA from
Antirrhinum majus, have been studied using paper chromatography, HPLC and
1H and
13C NMR spectroscopy involving the use of
1H
1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins
delphinidin-3-O-β-
d-(6-O-α-
l-
rhamnopyranosylglucopyranoside)-5-O-β-
d-[6-E (
and Z)>-p-
coumaroylglucopyranoside]
and
delphinidin-3-O-β-
d-
glucopyranoside-5-O-β-
d>-(6-O-E-p-
coumaroylglucopyranoside)
in addition to those found in the untransformed control plants. |
| doi_str_mv | 10.1016/0031-9422(96)00137-9 |
| format | article |
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O-glucosyltransferase cDNA from
Antirrhinum majus, have been studied using paper chromatography, HPLC and
1H and
13C NMR spectroscopy involving the use of
1H
1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins
delphinidin-3-O-β-
d-(6-O-α-
l-
rhamnopyranosylglucopyranoside)-5-O-β-
d-[6-E (
and Z)>-p-
coumaroylglucopyranoside]
and
delphinidin-3-O-β-
d-
glucopyranoside-5-O-β-
d>-(6-O-E-p-
coumaroylglucopyranoside)
in addition to those found in the untransformed control plants.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/0031-9422(96)00137-9</identifier><identifier>PMID: 8688182</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Anthocyanins - biosynthesis ; Anthocyanins - chemistry ; Anthocyanins - isolation & purification ; Biological and medical sciences ; Biotechnology ; Carbohydrate Conformation ; Carbohydrate Sequence ; Chromatography, High Pressure Liquid ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; Glucosyltransferases - biosynthesis ; Glucosyltransferases - metabolism ; Magnetic Resonance Spectroscopy ; Metabolism ; Molecular Sequence Data ; Molecular Structure ; Plant physiology and development ; Plants, Genetically Modified ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - metabolism ; Storage and secretion, pigments, phytochrome</subject><ispartof>Phytochemistry (Oxford), 1996-07, Vol.42 (4), p.1035-1038</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-928021eaba33ffa41901d44f370fd15b31fa31daa849b4a803c093caa4ced2c03</citedby><cites>FETCH-LOGICAL-c386t-928021eaba33ffa41901d44f370fd15b31fa31daa849b4a803c093caa4ced2c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3110556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8688182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Markham, Kenneth R.</creatorcontrib><title>Novel anthocyanins produced in petals of genetically transformed lisianthus</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose: flavonoid-3-
O-glucosyltransferase cDNA from
Antirrhinum majus, have been studied using paper chromatography, HPLC and
1H and
13C NMR spectroscopy involving the use of
1H
1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins
delphinidin-3-O-β-
d-(6-O-α-
l-
rhamnopyranosylglucopyranoside)-5-O-β-
d-[6-E (
and Z)>-p-
coumaroylglucopyranoside]
and
delphinidin-3-O-β-
d-
glucopyranoside-5-O-β-
d>-(6-O-E-p-
coumaroylglucopyranoside)
in addition to those found in the untransformed control plants.</description><subject>Anthocyanins - biosynthesis</subject><subject>Anthocyanins - chemistry</subject><subject>Anthocyanins - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Chromatography, High Pressure Liquid</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucosyltransferases - biosynthesis</subject><subject>Glucosyltransferases - metabolism</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Plant physiology and development</subject><subject>Plants, Genetically Modified</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - metabolism</subject><subject>Storage and secretion, pigments, phytochrome</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouq7-A4UeRPRQnWm6bXIRZPELF73oOcymiUa67Zq0C_vvTdllj55CmOcd3nkYO0O4QcDiFoBjKvMsu5LFNQDyMpV7bISi5CkvAfbZaIccseMQfgBgMimKQ3YoCiFQZCP2-tauTJ1Q0323ek2Na0Ky9G3Va1MlrkmWpqM6JK1NvkxjOqeprtdJ56kJtvWLCNUuuCHehxN2YCNsTrfvmH0-PnxMn9PZ-9PL9H6Wai6KLpWZgAwNzYlzaylHCVjluY2dbYWTOUdLHCsikct5TgK4Bsk1UR47ZRr4mF1u9saiv70JnVq4oE1dU2PaPqhSIOc8kxHMN6D2bQjeWLX0bkF-rRDU4FANgtQgSMnhEx2qIXa-3d_P44W70FZanF9s5xSiDxtlaBd2GEccNEfsboOZ6GLljFdBO9PEI5w3ulNV6_7v8QdxE435</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Markham, Kenneth R.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960701</creationdate><title>Novel anthocyanins produced in petals of genetically transformed lisianthus</title><author>Markham, Kenneth R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-928021eaba33ffa41901d44f370fd15b31fa31daa849b4a803c093caa4ced2c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Anthocyanins - biosynthesis</topic><topic>Anthocyanins - chemistry</topic><topic>Anthocyanins - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Chromatography, High Pressure Liquid</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucosyltransferases - biosynthesis</topic><topic>Glucosyltransferases - metabolism</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>Plant physiology and development</topic><topic>Plants, Genetically Modified</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - metabolism</topic><topic>Storage and secretion, pigments, phytochrome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markham, Kenneth R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markham, Kenneth R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel anthocyanins produced in petals of genetically transformed lisianthus</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>42</volume><issue>4</issue><spage>1035</spage><epage>1038</epage><pages>1035-1038</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose: flavonoid-3-
O-glucosyltransferase cDNA from
Antirrhinum majus, have been studied using paper chromatography, HPLC and
1H and
13C NMR spectroscopy involving the use of
1H
1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins
delphinidin-3-O-β-
d-(6-O-α-
l-
rhamnopyranosylglucopyranoside)-5-O-β-
d-[6-E (
and Z)>-p-
coumaroylglucopyranoside]
and
delphinidin-3-O-β-
d-
glucopyranoside-5-O-β-
d>-(6-O-E-p-
coumaroylglucopyranoside)
in addition to those found in the untransformed control plants.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>8688182</pmid><doi>10.1016/0031-9422(96)00137-9</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9422 |
| ispartof | Phytochemistry (Oxford), 1996-07, Vol.42 (4), p.1035-1038 |
| issn | 0031-9422 1873-3700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78133329 |
| source | Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list) |
| subjects | Anthocyanins - biosynthesis Anthocyanins - chemistry Anthocyanins - isolation & purification Biological and medical sciences Biotechnology Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid DNA, Complementary Fundamental and applied biological sciences. Psychology Glucosyltransferases - biosynthesis Glucosyltransferases - metabolism Magnetic Resonance Spectroscopy Metabolism Molecular Sequence Data Molecular Structure Plant physiology and development Plants, Genetically Modified Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Storage and secretion, pigments, phytochrome |
| title | Novel anthocyanins produced in petals of genetically transformed lisianthus |
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