DNAse I sensitivity of Microtus agrestis active, inactive and reactivated X chromosomes in mouse-Microtus cell hybrids

We isolated Microtus agrestis-mouse somatic cell hybrid clones which had retained either the active or the inactive M. agrestis X chromosome. In both hybrid clones the X chromosomes retained their original chromatin conformation as studied by the in situ nick translation technique--the active X chro...

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Bibliographic Details
Published in:Chromosoma 1988-01, Vol.96 (3), p.227-230
Main Authors: KEREM, B.-S, KOTTUSCH-GEISELER, V, KALSCHEUER, V, GOITEIN, R, SPERLING, K, MARCUS, M
Format: Article
Language:English
Subjects:
Animals
Arvicolinae - genetics
Biological and medical sciences
Chromatin - drug effects
Chromatin - ultrastructure
Cytogenetics
Deoxyribonuclease I - pharmacology
Dosage Compensation, Genetic
Euchromatin
Female
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Hybrid Cells - ultrastructure
Hypoxanthine Phosphoribosyltransferase - genetics
Mice
microtus agrestis
Vertebrata
X Chromosome - drug effects
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Summary:We isolated Microtus agrestis-mouse somatic cell hybrid clones which had retained either the active or the inactive M. agrestis X chromosome. In both hybrid clones the X chromosomes retained their original chromatin conformation as studied by the in situ nick translation technique--the active X chromosome retained its high sensitivity to DNase I while the inactive one remained insensitive. A clone in which the hypoxanthine guanine phosphoribosyltransferase (HPRT) gene had been spontaneously reactivated was isolated from the hybrid containing the inactive X chromosome. The in situ nick translation technique was used to study possible DNA conformation changes in the euchromatin of the inactive X chromosome with special reference to the reactivated HPRT locus. We found that the euchromatin in this X chromosome exhibited the same low sensitivity to DNase I as is characteristic of the inactive X chromosome.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00302362