Comparative efficiency of simple lipopeptide constructs for in vivo induction of virus-specific CTL

We have previously shown that virus-specific CTL responses can be elicited in vivo by injecting, without adjuvant, 12–40 amino acid-long peptides, modified in C-terminal position by a simple lipidic amino acid. In this paper, we have studied the chemical accessibility, and the ability to induce in m...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 1996-04, Vol.14 (5), p.375-382
Main Authors: Deprez, Benoît, Sauzet, Jean-Pierre, Boutillon, Christophe, Martinon, Frédéric, Tartar, André, Sergheraert, Christian, Guillet, Jean-Gérard, Gomard, Elisabeth, Gras-Masse, Hélène
Format: Article
Language:English
Subjects:
AIDS/HIV
Amino Acid Sequence
Animals
Biological and medical sciences
Cytotoxic T lymphocytes
Fundamental and applied biological sciences. Psychology
Gene Products, env - immunology
HIV-1 - immunology
human immunodeficiency virus 1
lipopeptides
Lipoproteins - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred DBA
Microbiology
Molecular Sequence Data
Peptide Fragments - immunology
synthetic vaccines
T-Lymphocytes, Cytotoxic - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
Viral Vaccines - immunology
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have previously shown that virus-specific CTL responses can be elicited in vivo by injecting, without adjuvant, 12–40 amino acid-long peptides, modified in C-terminal position by a simple lipidic amino acid. In this paper, we have studied the chemical accessibility, and the ability to induce in mice a CTL response, of a series of lipopeptides derived from the HIV-1 env (312–327) or (302–335) sequences. We showed that a single modification of these peptides by a lipidic amino acid, preferably in C-terminal position, results in the ability to reproducibly induce, without adjuvant, a relevant CTL response. No clear discrimination appeared concerning the nature of the lipidic modification. Our findings indicate that modification of a relatively long peptide by a N ε-palmitoyl-L-Lysylamide can be achieved by conventional methods of synthesis and characterization, offering the possibility to develop low-cost synthetic vaccines in models in which the CTL component is of importance.
ISSN:0264-410X
1873-2518
DOI:10.1016/0264-410X(95)00220-U