A new method for studying the incorporation of nonesterified fatty acids into cardiac lipids by using deuterium-labelled palmitate

A new method for measuring the incorporation of exogenous fatty acids into myocardial lipids of working rat hearts using deuterium-labelled palmitate has been developed. After perfusing isolated hearts, the fatty acid composition of triglycerides, diglycerides, monoglycerides, cholesterol esters, an...

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Bibliographic Details
Published in:Basic research in cardiology 1988, Vol.83 (1), p.87-93
Main Authors: HÜTTER, J. F, SCHWEICKHARDT, C, HUNNEMAN, D. H, PIPER, H. M, SPIECKERMANN, P. G
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cholesterol Esters - metabolism
Deuterium
Diglycerides - metabolism
Fatty Acids, Nonesterified - metabolism
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Glycerides - metabolism
Heart
Lipid Metabolism
Myocardium - metabolism
Palmitic Acid
Palmitic Acids - metabolism
Rats
Rats, Inbred Strains
Triglycerides - metabolism
Vertebrates: cardiovascular system
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Summary:A new method for measuring the incorporation of exogenous fatty acids into myocardial lipids of working rat hearts using deuterium-labelled palmitate has been developed. After perfusing isolated hearts, the fatty acid composition of triglycerides, diglycerides, monoglycerides, cholesterol esters, and nonesterified fatty acids (NEFA) was measured by a mass fragmentographic method. Hearts perfused only with 5 mM glucose and 1 mM lactate perfusion as basic substrates showed a significant decrease of triglyceride content, while the other lipids were not found to be significantly reduced. The fatty acid composition of all lipids were not affected. An addition of D31-palmitate complexed to albumin at a molar ratio of 5:1, caused a dose-dependent incorporation into triglycerides and diglycerides which suggested saturation kinetics. The tissue content of nonesterified D31-palmitate was found to be linearly related to its concentration in the perfusate. It may be of note that the concentration of the other NEFA was significantly affected neither by a fatty-acid-free perfusion nor by a perfusion with D31-palmitate. A significant incorporation of the supplied fatty acid into cholesterol esters and monoglycerides could not be detected.
ISSN:0300-8428
1435-1803
DOI:10.1007/BF01907108