Microwaves and the Cell Membrane: IV. Protein Shedding in the Human Erythrocyte: Quantitative Analysis by High-Performance Liquid Chromatography

• Published in: Radiation research 1988-06, Vol.114 (3), p.500-514
• Main Authors: Liburdy, R. P., Rowe, A. W., Vanek, P. F.
• Format: Article
• Language: English
• Subjects: Blood group antigens; Blood Proteins - metabolism; Cell membranes; Chromatography, High Pressure Liquid; Elution; Erythrocyte membrane; Erythrocyte Membrane - radiation effects; Erythrocytes; Humans; Liposomes; Liquid chromatography; Membrane proteins; Membrane Proteins - metabolism; Microwaves; Space life sciences; Transition temperature
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3577121

The erythrocyte responds to microwave fields by shedding at least 11 low-molecular-weight proteins of ≤31,000 Da, with components of 28,000-31,000 Da released during the destabilization of divalent calcium-protein bridges [R. P. Liburdy and P. F. Vanek, Radiat. Res. 109, 382-395 (1987)]. Significantly, protein shedding was shown to be restricted to exposure temperatures coinciding with the cell membrane phase/structural transition temperature, $T_{{\rm c}}$, of 17-25°C. We report here a further characterization of protein shedding at $T_{{\rm c}}$ using high-performance liquid chromatography and membrane-associated blood group antigen testing. Proteins shed from human erythocytes in microwave fields (2450 MHz, CW) compared to sham-heating displayed a twofold increase in total protein mass released concomitant with the appearance of unique protein species during reverse-phase, hydrophobic interaction, and anion-exchange HPLC. These HPLC analyses indicate that microwaves result in the shedding of proteins which are relatively nonpolar and hydrophobic and which carry a net positive electrostatic charge compared to those released during sham-heat treatment. Assessment of 23 blood group antigens that represent integral protein markers on the erythrocyte cell surface indicates that microwave fields do not result in the exhaustive loss of these proteins. The class of proteins that is shed in response to microwave fields most likely is the loosely bound "peripheral" or extrinsic proteins associated with the exterior of the cell surface. Such proteins play a major role in the transduction of signals to integral membrane proteins which span the bilayer. That this class of proteins is susceptible to release by microwave fields is discussed in relation to microwave absorption at the cell surface by membrane-associated bound water, field interaction with dipolar side groups, and the disruption of divalent cation bridges known to stabilize peripheral membrane proteins.