Homeodomain interaction with the beta subunit of the general transcription factor TFIIE

Homeodomain-containing proteins play a crucial role as transcriptional regulators in the process of cell differentiation. The homeodomain performs a dual function in this regard, acting as a DNA binding domain and participating in protein-protein interactions that enhance DNA binding specificity or...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-08, Vol.271 (35), p.20993-20996
Main Authors: Zhu, A, Kuziora, M.A
Format: Article
Language:English
Subjects:
ABDOMINAL-B PROTEIN
ACILTRANSFERASA
ACYLTRANSFERASE
ACYLTRANSFERASES
ADN
ANTENNAPEDIA PROTEIN
Base Sequence
BINDING
BINDING PROTEINS
BINDING SITES
Cell Line
CHLORAMPHENICOL ACETYLTRANSFERASE
DNA
DNA BINDING MOTIFS
DNA Primers
DROSOPHILA MELANOGASTER
EXPRESION GENICA
EXPRESSION DES GENES
FACTEUR DE TRANSCRIPTION
FACTORES DE TRANSCRIPCION
GENE
GENE EXPRESSION
GENES
GENETIC REGULATION
GENETICA
GENETICS
GENETIQUE
Glutathione Transferase - metabolism
HeLa Cells
HOMEODOMAIN PROTEINS
Homeodomain Proteins - metabolism
Humans
Molecular Sequence Data
Protein Binding
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
Recombinant Fusion Proteins - metabolism
REPORTER GENES
TRANSACTIVATION
TRANSCRIPCION
TRANSCRIPTION
TRANSCRIPTION FACTORS
Transcription Factors - metabolism
Transcription Factors, TFII
Transcription, Genetic
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Summary:Homeodomain-containing proteins play a crucial role as transcriptional regulators in the process of cell differentiation. The homeodomain performs a dual function in this regard, acting as a DNA binding domain and participating in protein-protein interactions that enhance DNA binding specificity or regulatory activity. Here we describe a homeodomain class-specific interaction with the beta subunit of the general transcription factor TFIIE. We show that the Antennapedia and Abdominal-B homeodomains bind to TFIIEbeta, but the even-skipped homeodomain does not. Using a two-hybrid assay performed in cultured cells, we demonstrate that the homeodomain-TFIIEbeta interaction occurs in vivo. The Abdominal-B homeodomain is shown to activate transcription in vitro, and this activation can be blocked with anti-TFIIEbeta antibody without affecting basal transcription levels. Together with published data demonstrating an interaction between proteins containing even-skipped class homeodomains and the TATA-binding protein (Um, M., Li, C., and Manley, J.L. (1995) Mol. Cell. Biol. 15, 5007-5016; Zhang, H., Catron, K. M., and Abate-Shen, C. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 1764-1769), these results suggest various homeodomain containing proteins interact with different general transcription factors, an observation that may have important implications for transcriptional regulation
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.35.20993