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The e subunit gene of murine F1F0-ATP synthase: genomic sequence, chromosomal mapping, and diet regulation
Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence diffe...
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| Published in: | The Journal of biological chemistry 1996-08, Vol.271 (34), p.20942-20948 |
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| container_end_page | 20948 |
| container_issue | 34 |
| container_start_page | 20942 |
| container_title | The Journal of biological chemistry |
| container_volume | 271 |
| creator | Swartz, D.A. (University of Illinois, Urbana, IL.) Park, E.I Visek, W.J Kaput, J |
| description | Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near DCMit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1.1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p 0.05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. The implications of the high degree of sequence similarity to the e subunit for rat and bovine F1F0-ATP synthase and its regulation by diet are discussed |
| doi_str_mv | 10.1074/jbc.271.34.20942 |
| format | article |
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(University of Illinois, Urbana, IL.) ; Park, E.I ; Visek, W.J ; Kaput, J</creator><creatorcontrib>Swartz, D.A. (University of Illinois, Urbana, IL.) ; Park, E.I ; Visek, W.J ; Kaput, J</creatorcontrib><description>Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near DCMit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1.1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p 0.05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. 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(University of Illinois, Urbana, IL.)</creatorcontrib><creatorcontrib>Park, E.I</creatorcontrib><creatorcontrib>Visek, W.J</creatorcontrib><creatorcontrib>Kaput, J</creatorcontrib><title>The e subunit gene of murine F1F0-ATP synthase: genomic sequence, chromosomal mapping, and diet regulation</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near DCMit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1.1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p 0.05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. The implications of the high degree of sequence similarity to the e subunit for rat and bovine F1F0-ATP synthase and its regulation by diet are discussed</description><subject>ACEITE DE MAIZ</subject><subject>ALIMENTACION</subject><subject>ALIMENTATION</subject><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>CARTE GENETIQUE</subject><subject>Chromosome Mapping</subject><subject>COEUR</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>CONSOMMATION ALIMENTAIRE</subject><subject>CONSUMO DE ALIMENTOS</subject><subject>CORAZON</subject><subject>CORPS GRAS</subject><subject>Diet</subject><subject>DIETA</subject><subject>Eating</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FOIE</subject><subject>GENE</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>GENES</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>GRASAS</subject><subject>HIDROLASAS</subject><subject>HIGADO</subject><subject>HUILE DE MAIS</subject><subject>HYDROLASE</subject><subject>INANICION</subject><subject>INANITION</subject><subject>Liver - enzymology</subject><subject>MAPAS GENETICOS</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Myocardium - enzymology</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Proton-Translocating ATPases - genetics</subject><subject>RATON</subject><subject>REGIME ALIMENTAIRE</subject><subject>RNA, Messenger - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>SOURIS</subject><subject>Time Factors</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNotkM1Lw0AQxRdRaq3eRRD25KmJu5tkP7yVYlUoKNiCt7CbzKZbkmzNJof-90baucxj3o_HYxC6pySmRKTPe1PETNA4SWNGVMou0JQSmURJRn8u0ZQQRiPFMnmNbkLYk3FSRSdoIgVhMkumaL_ZAQYcBjO0rscVtIC9xc3QuVGt6IpEi80XDse23-kAL_-Eb1yBA_wO0BYwx8Wu840PvtE1bvTh4NpqjnVb4tJBjzuohlr3zre36MrqOsDdec_QdvW6Wb5H68-3j-ViHVnGsj6SGXBjLdOFYYkQkkkuQGlBFSSZksZyQwkvFLXjzUjBJS9NoVJtuU2FhWSGnk65h86PHUOfNy4UUNe6BT-EfIzkSio-go9ncDANlPmhc43ujvn5OaP_cPKt9rmuOhfy7bcSKWeMJH_EhG80</recordid><startdate>19960823</startdate><enddate>19960823</enddate><creator>Swartz, D.A. 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(University of Illinois, Urbana, IL.) ; Park, E.I ; Visek, W.J ; Kaput, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f225t-85e6bff2acb237782867e9a719e3598bf6b106c91fa71b87686dbc94af6f47fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ACEITE DE MAIZ</topic><topic>ALIMENTACION</topic><topic>ALIMENTATION</topic><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>CARTE GENETIQUE</topic><topic>Chromosome Mapping</topic><topic>COEUR</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>CONSOMMATION ALIMENTAIRE</topic><topic>CONSUMO DE ALIMENTOS</topic><topic>CORAZON</topic><topic>CORPS GRAS</topic><topic>Diet</topic><topic>DIETA</topic><topic>Eating</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FOIE</topic><topic>GENE</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>GENES</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>GRASAS</topic><topic>HIDROLASAS</topic><topic>HIGADO</topic><topic>HUILE DE MAIS</topic><topic>HYDROLASE</topic><topic>INANICION</topic><topic>INANITION</topic><topic>Liver - enzymology</topic><topic>MAPAS GENETICOS</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Sequence Data</topic><topic>Myocardium - enzymology</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Proton-Translocating ATPases - genetics</topic><topic>RATON</topic><topic>REGIME ALIMENTAIRE</topic><topic>RNA, Messenger - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>SOURIS</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swartz, D.A. (University of Illinois, Urbana, IL.)</creatorcontrib><creatorcontrib>Park, E.I</creatorcontrib><creatorcontrib>Visek, W.J</creatorcontrib><creatorcontrib>Kaput, J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swartz, D.A. (University of Illinois, Urbana, IL.)</au><au>Park, E.I</au><au>Visek, W.J</au><au>Kaput, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The e subunit gene of murine F1F0-ATP synthase: genomic sequence, chromosomal mapping, and diet regulation</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1996-08-23</date><risdate>1996</risdate><volume>271</volume><issue>34</issue><spage>20942</spage><epage>20948</epage><pages>20942-20948</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near DCMit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1.1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p 0.05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. The implications of the high degree of sequence similarity to the e subunit for rat and bovine F1F0-ATP synthase and its regulation by diet are discussed</abstract><cop>United States</cop><pmid>8702853</pmid><doi>10.1074/jbc.271.34.20942</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1996-08, Vol.271 (34), p.20942-20948 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | ACEITE DE MAIZ ALIMENTACION ALIMENTATION Animals ARN MENSAJERO ARN MESSAGER Base Sequence CARTE GENETIQUE Chromosome Mapping COEUR COMPOSICION QUIMICA COMPOSITION CHIMIQUE CONSOMMATION ALIMENTAIRE CONSUMO DE ALIMENTOS CORAZON CORPS GRAS Diet DIETA Eating EXPRESION GENICA EXPRESSION DES GENES FOIE GENE Gene Expression Regulation, Enzymologic GENES GENETICA GENETIQUE GRASAS HIDROLASAS HIGADO HUILE DE MAIS HYDROLASE INANICION INANITION Liver - enzymology MAPAS GENETICOS Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Myocardium - enzymology Polymorphism, Restriction Fragment Length Proton-Translocating ATPases - genetics RATON REGIME ALIMENTAIRE RNA, Messenger - genetics SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE SOURIS Time Factors |
| title | The e subunit gene of murine F1F0-ATP synthase: genomic sequence, chromosomal mapping, and diet regulation |
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