Molecular genetic methods for improving secondary-metabolite production in actinomycetes

The practical applications of genetic engineering to improve secondary-metabolite production in actinomycetes are potentially numerous, but have been limited by: (1) restriction barriers, which can hinder the introduction of DNA into many actinomycetes; (2) self-replicating plasmid-cloning vectors,...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 1996-07, Vol.14 (7), p.245-250
Main Authors: Baltz, Richard H., Hosted, Thomas J.
Format: Article
Language:English
Subjects:
Actinomycetales - genetics
Actinomycetales - metabolism
Biological and medical sciences
Biotechnology
DNA Transposable Elements
DNA, Recombinant - genetics
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic Engineering - methods
Genetic Markers
Genetic technics
Genome, Bacterial
Methods. Procedures. Technologies
Miscellaneous
Molecular Biology - methods
Plasmids - genetics
Recombination, Genetic
Synthetic digonucleotides and genes. Sequencing
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Summary:The practical applications of genetic engineering to improve secondary-metabolite production in actinomycetes are potentially numerous, but have been limited by: (1) restriction barriers, which can hinder the introduction of DNA into many actinomycetes; (2) self-replicating plasmid-cloning vectors, which generally inhibit secondary-metabolite production; and (3) recombinants containing heterologous DNA, which may be subject to additional regulatory hurdles. Recently, intergeneric conjugation has been used to circumvent host restriction, and integration of cloned DNA into neutral genomic sites prevents product inhibition by self-replicating plasmids, and has enabled construction of recombinant strains lacking heterologous DNA sequences. The rpsL system permits direct selection for gene replacements and gene insertions that can facilitate this process.
ISSN:0167-7799
1879-3096
DOI:10.1016/0167-7799(96)10034-2