Transformation of gypsy moth (Lymantria dispar) cell lines by infection with Glyptapanteles indiensis polydnavirus

Glyptapanteles indiensis, a species of braconid parasitic wasp, infects its host Lymantria dispar (gypsy moth) with a polydnavirus (GiPDV) to suppress the host immune system during parasitization. Here it is shown that GiPDV can infect L dispar cell lines and that a portion of the GiPDV genome is st...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-08, Vol.225 (3), p.764-770
Main Authors: McKelvey, T.A. (USDA, ARS, Insect Biocontrol Laboratory, Beltsville, MD.), Lynn, D.E, Gundersen-Rindal, D, Guzo, D, Stoltz, D.A, Guthrie, K.P, Taylor, P.B, Dougherty, E.M
Format: Article
Language:English
Subjects:
ADN
Animals
BRACONIDAE
Cell Line
Cell Transformation, Viral - genetics
CONTROL BIOLOGICO
CULTIVO DE CELULAS
CULTURE DE CELLULE
DNA, Viral - genetics
Genetic Vectors
INFECCION
INFECTION
INSECTE NUISIBLE
INSECTOS DANINOS
LUTTE BIOLOGIQUE
LYMANTRIA DISPAR
Moths - parasitology
Moths - virology
ORGANISME TRANSGENIQUE
PARASITE
PARASITOS
Polydnaviridae - genetics
Polydnaviridae - pathogenicity
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
TRANSGENICOS
VIRUS DE INVERTEBRADOS
VIRUS DES INVERTEBRES
Wasps - pathogenicity
Wasps - virology
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Description
Summary:Glyptapanteles indiensis, a species of braconid parasitic wasp, infects its host Lymantria dispar (gypsy moth) with a polydnavirus (GiPDV) to suppress the host immune system during parasitization. Here it is shown that GiPDV can infect L dispar cell lines and that a portion of the GiPDV genome is stably maintained in infected cells. Results of Southern hybridization analyses suggested that this portion of the GiPDV genome is integrated into the L. dispar cellular genome. This is the first report of an insect viral DNA molecule that can apparently integrate into lepidopteran insect cells
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1248