Chromosomal Assignment of Four Testis-Expressed Mouse Genes from a New Family of Transmembrane Proteins (ADAMs) Involved in Cell–Cell Adhesion and Fusion

A new gene family of multidomain membrane proteins (ADAMs) that includeADisintegrinAndMetalloprotease domain comprises an increasing number of identified members. Two members of this family, fertilin α and fertilin β, form a heterodimeric protein that is required for sperm–egg fusion. Most recently,...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-06, Vol.34 (3), p.413-417
Main Authors: Cho, Chunghee, Primakoff, Paul, White, Judith M., Myles, Diana G.
Format: Article
Language:English
Subjects:
ADAM Proteins
Animals
Cell Adhesion
Cell Fusion
Chromosome Mapping
Crosses, Genetic
Female
Fertilins
Genetic Markers
Humans
Male
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - genetics
Metalloendopeptidases - biosynthesis
Metalloendopeptidases - genetics
Mice
Mice, Inbred C57BL
Multigene Family
Muridae
Polymorphism, Restriction Fragment Length
Testis - metabolism
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Summary:A new gene family of multidomain membrane proteins (ADAMs) that includeADisintegrinAndMetalloprotease domain comprises an increasing number of identified members. Two members of this family, fertilin α and fertilin β, form a heterodimeric protein that is required for sperm–egg fusion. Most recently, it has been shown that a third family member, meltrin α, is involved in myoblast fusion (Yagami-Hiromasaet al.,1995,Nature377: 652–656). Using restriction fragment length polymorphism analysis of a DNA panel from an interspecific backcross, we have determined the chromosomal locations of four mouse genes of this family that are expressed in testis: fertilin α, fertilin β, ADAM 4, and ADAM 5. These genes have been given the locus symbolsFtna(fertilin α),Ftnb(fertilin β),Adam4(ADAM 4), andAdam5(ADAM 5). They were mapped to chromosomes 5, 14, 9, and 8, respectively, revealing a dispersed localization. Human chromosome locations of these genes are predicted on the basis of the mapping results using the information provided by comparative linkage maps. Because all four of these ADAM genes are expressed in testis and fertilin α and β have been found to be important for fertilization, we compared their chromosomal locations with known mouse mutations affecting spermatogenesis and fertility.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0305