Use of serum-free, compositionally defined medium for analysis of macrophage differentiation in vitro

Macrophage differentiation is mediated by the action of a variety of environmental signals, such as cytokines and endotoxin. For in vitro analysis of macrophage differentiation, most studies utilize a basal tissue culture medium supplemented with fetal calf serum (FCS). As the composition of specifi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of leukocyte biology 1988-08, Vol.44 (2), p.136-142
Main Authors: VOGEL, S. N, PIN YU PERERA, HOGAN, M. M, MAJDE, J. A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Differentiation - drug effects
Cells, Cultured
Culture Media - analysis
Culture Media - pharmacology
Cytotoxicity, Immunologic - drug effects
Dexamethasone - pharmacology
Female
Fetal Blood - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Antigens Class II - biosynthesis
Immunobiology
Interferons - pharmacology
Macrophage Activation - drug effects
Macrophages - cytology
Macrophages - drug effects
Macrophages - immunology
Mice
Mice, Inbred C3H
Myeloid cells: ontogeny, maturation, markers, receptors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Macrophage differentiation is mediated by the action of a variety of environmental signals, such as cytokines and endotoxin. For in vitro analysis of macrophage differentiation, most studies utilize a basal tissue culture medium supplemented with fetal calf serum (FCS). As the composition of specific components within FCS varies enormously from lot to lot, one can never be certain that the differentiative effects observed in vitro are attributable solely to the exogenous signals provided to the cultures. In this study, primary macrophages were cultured in a basal medium supplemented either with FCS or a compositionally defined supplement, HL-1, and a spectrum of differentiative functions (i.e., induction of antiviral activity, Fc receptor-mediated phagocytosis, Ia antigen expression, and tumoricidal activity) were measured following stimulation with exogenous signals. The results indicate that the use of serum-free, defined media may provide an important approach to dissect and characterize the differentiative signals which are operative in macrophage activation.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.44.2.136