Effect of hypotaurine on in vitro fertilization and production of term offspring from in vitro-fertilized ova of the Japanese field vole, Microtus montebelli

The present study was proposed to establish new in vitro fertilization (IVF)-embryo transfer techniques for wild mammals. We demonstrated that IVF of the Japanese field vole, Microtus montebelli, is improved by hypotaurine, and that IVF vole embryos can develop to normal offspring after transfer to...

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Bibliographic Details
Published in:Biology of reproduction 1996-03, Vol.54 (3), p.625-630
Main Authors: Wakayama, T. (University of Tokyo, Tokyo, Japan.), Suto, J, Imamura, K, Toyoda, Y, Kurohmaru, M, Hayashi, Y
Format: Article
Language:English
Subjects:
ACIDE AMINE
AMINOACIDOS
Animal productions
Animals
Arvicolinae
Biological and medical sciences
Blastocyst - physiology
CAMPAGNOL
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
Embryo Transfer
Embryonic and Fetal Development
ESPERMATOZOO
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
FECONDATION
FECUNDACION
Female
FERTILIDAD
FERTILITE
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Male
MEDIO DE CULTIVO
MILIEU DE CULTURE
OVULE
OVULO
Pregnancy
Spermatozoa - drug effects
Spermatozoa - physiology
SPERMATOZOIDE
Taurine - analogs & derivatives
Taurine - pharmacology
Terrestrial animal productions
TOPILLOS
TRANSFERENCIA DE EMBRIONES
TRANSFERT EMBRYONNAIRE
Vertebrates
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Summary:The present study was proposed to establish new in vitro fertilization (IVF)-embryo transfer techniques for wild mammals. We demonstrated that IVF of the Japanese field vole, Microtus montebelli, is improved by hypotaurine, and that IVF vole embryos can develop to normal offspring after transfer to recipient females. The rate of IVF was significantly increased when 0.1 or 1 mM of hypotaurine was added during sperm preincubation and fertilization (p 0.05). Preincubation of spermatozoa with 1 mM hypotaurine prior to oocyte insemination did not have a significant effect on fertilization rate (p 0.01). At 96 h after insemination, most cultured embryos developed to the 2-cell stage (68-83%), but development to blastocysts was very low (0-3%) for all treatments. Although 43 living offspring were produced after transfer of IVF pronuclear embryos to recipients, the delivery rate of recipients was very low (21%). The litter size of pseudopregnant recipients that produced a litter was 4-11, and the rate of in vivo development of transferred embryos in the pregnant recipients was 41%. These results suggest that hypotaurine affects IVF rather then sperm preincubation and that IVF embryos can develop to normal offspring
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod54.3.625