In Vitro Sequence-Specific Cleavage in Transcribed Spacer of Mouse Precursor Ribosomal RNA

When we compared the sequences near four terminal sites of transcripts including two endoribonucleolytic cleavage sites in external transcribed spacer 1 upstream of the 5' end of the mouse 18S rRNA (Mishima, Y., Mitsuma, T., & Ogata, K. (1985) EMBO J. 4, 3879-3886), a seven-nucleotide conse...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1988, Vol.103 (6), p.992-997
Main Authors: Nashimoto, Masayuki, Ogata, Kikuo, Mishima, Yukio
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
DNA - analysis
Fundamental and applied biological sciences. Psychology
Gene expression
In Vitro Techniques
Mice
Molecular and cellular biology
Molecular genetics
Peptide Mapping
RNA Precursors - genetics
Transcription, Genetic
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:When we compared the sequences near four terminal sites of transcripts including two endoribonucleolytic cleavage sites in external transcribed spacer 1 upstream of the 5' end of the mouse 18S rRNA (Mishima, Y., Mitsuma, T., & Ogata, K. (1985) EMBO J. 4, 3879-3886), a seven-nucleotide consensus sequence, GGPyUUGPy (Py is C or U), was obtained. The results of both in vitro pulse-chase experiments and S1 nuclease mappings using the mouse rDNA fragment of the transcription initiation region indicated that ribonucleolytic cleavages take place in the sequence matching the consensus sequence at more than five nucleotides out of the seven positions. Furthermore, effective ribonucleolytic cleavages in vitro were observed in a sequence, GGCUUGU, in the internal transcribed spacer 2 located between 5.8S and 28S rRNA. These results demonstrate that the ribonucleolytic cleavages occur preferentially in the sequence of GGPyUUGPy in the transcribed spacer regions of the mouse pre-rRNA. From this information, we infer the existence of processing steps for the pre-rRNA maturation involving the sequence-specific ribonucleolytic cleavages.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a122399