Penicillin G—Induced Microbicidal Activity of Endothelial Cells Cultured on Gelfoam Blocks

A body of evidence has surfaced documenting the ability of endothelial cells cultured on mono-layers to phagocytose but not kill bacteria. Several years ago, a new three-dimensional endothelial cell culturing model was developed, which simulated the morphology of the endothelium in small vessels and...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1996-11, Vol.174 (5), p.1001-1009
Main Authors: Zhang, Bin, Centra, Michelle, Cao, Guan Liang, Taylor, Robert M., Ratych, Roman E., Rosen, Gerald M.
Format: Article
Language:English
Subjects:
Absorbable gelatin sponges
Animals
Antibiotics
Bacteria
Cattle
Cell growth
Cells, Cultured
Cultured cells
Cytochalasin B - pharmacology
Cytochalasins
Endothelial cells
Endothelium, Vascular - cytology
Female
Gelatin Sponge, Absorbable - pharmacology
Major Articles
Penicillin
Penicillin G - pharmacology
Penicillins - pharmacology
Phagocytosis
Pregnancy
Staphylococcus aureus - immunology
Streptomycin - pharmacology
Ungulates
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A body of evidence has surfaced documenting the ability of endothelial cells cultured on mono-layers to phagocytose but not kill bacteria. Several years ago, a new three-dimensional endothelial cell culturing model was developed, which simulated the morphology of the endothelium in small vessels and capillaries. Given that endothelial cellsmay be derived from the same pluripotent stem cells as macrophages, the question of whether endothelial cells might phagocytose and kill bacteria was explored. Endothelial cells grown on Gelfoam blocks exhibited bactericidal activity towards Staphylococcus aureus, reaching maximal killing of >90% after 2 h. Evidence documents the involvementof bacterial adherence to the plasma membrane of the endothelial cell. This is followed by phagocytosis of S. aureus, leading to intracellular killing. Penicillin G, included in the endothelial cell growth medium, was found to be a critical factor in the bactericidal activity demonstrated by Gelfoam blocks laden with endothelial cells.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/174.5.1001