Structural changes of nucleosomal particles and isolated core-histone octamers induced by chemical modification of lysine residues

Treatment of nucleosomal particles and isolated core-histone octamers with dimethylmaleic anhydride, but not with acetic anhydride, is accompanied by a biphasic release of the two H2A.H2B dimers, the first dimer being more easily released than the second. With both kinds of particles, 50% of histone...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 1988-07, Vol.27 (15), p.5635-5640
Main Authors: Nieto, Maria Angela, Palacian, Enrique
Format: Article
Language:English
Subjects:
Acetic Anhydrides
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chemical Phenomena
Chemistry
Chickens
Fundamental and applied biological sciences. Psychology
Histones
Holoproteins
In Vitro Techniques
Lysine
Macromolecular Substances
Maleic Anhydrides
Nuclear proteins
Nucleosomes - drug effects
Nucleosomes - ultrastructure
Protein Binding - drug effects
Proteins
Sodium Chloride - pharmacology
Structure-Activity Relationship
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Treatment of nucleosomal particles and isolated core-histone octamers with dimethylmaleic anhydride, but not with acetic anhydride, is accompanied by a biphasic release of the two H2A.H2B dimers, the first dimer being more easily released than the second. With both kinds of particles, 50% of histones H2A and H2B are released for modification of approximately 35% of the histone amino groups. The similar behavior of nucleosomal particles and isolated core-histone octamers is consistent with the same structure of the histone octamer in the nucleosomal particle and in the free octamer in 2 M NaCl. The described release of H2A.H2B dimers allows the preparation of nucleosomal particles deficient in one H2A.H2B dimer and of the histone hexamers H2A.H2B.(H3.H4)2. For more extensive modifications, both reagents, acetic and dimethylmaleic anhydrides, cause the dissociation of nucleosomal particles with liberation of double-stranded DNA, which suggests that lysine amino groups are involved in the binding of histones to DNA. The modified nucleosomal particles are more sensitive to ionic strength than those untreated, and the presence of salt (NaCl) increases the extent of DNA release. The histones corresponding to the liberated DNA, except H2A and H2B released with dimethylmaleic anhydride, are apparently bound to the DNA-containing particles as extra histones.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00415a036