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Morphological characterization of bovine primordial follicles and their environment in vivo
Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 2...
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| Published in: | Biology of reproduction 1996-11, Vol.55 (5), p.1003-1011 |
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| Language: | English |
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| container_end_page | 1011 |
| container_issue | 5 |
| container_start_page | 1003 |
| container_title | Biology of reproduction |
| container_volume | 55 |
| creator | Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.) Rodgers, R.J |
| description | Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 micromolar thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microgram), breadth (26.8 +/- 1.5 micrometer), and depth ( 30.4 +/- 1.4 micrometer) (mean +/- SEM; n |
| doi_str_mv | 10.1095/biolreprod55.5.1003 |
| format | article |
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This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 micromolar thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microgram), breadth (26.8 +/- 1.5 micrometer), and depth ( 30.4 +/- 1.4 micrometer) (mean +/- SEM; n</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod55.5.1003</identifier><identifier>PMID: 8902210</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cell Nucleus - ultrastructure ; CELLULE ; CELULAS ; CHIMIE ; Cytoplasm - ultrastructure ; DIMENSION ; ESTRUCTURA CELULAR ; Female ; Fundamental and applied biological sciences. Psychology ; Granulosa Cells - ultrastructure ; IMMUNOLOGIE ; INMUNOLOGIA ; Mammalian female genital system ; Microscopy, Electron ; Morphology. Physiology ; Oocytes - ultrastructure ; OVAIRE ; Ovarian Follicle - ultrastructure ; OVARIOS ; Ovary - blood supply ; Ovary - cytology ; QUIMICA ; Stem Cells - ultrastructure ; STRUCTURE CELLULAIRE ; ULTRAESTRUCTURA ; ULTRASTRUCTURE ; VACA ; VACHE ; Vertebrates: reproduction ; von Willebrand Factor - analysis</subject><ispartof>Biology of reproduction, 1996-11, Vol.55 (5), p.1003-1011</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3249731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8902210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.)</creatorcontrib><creatorcontrib>Rodgers, R.J</creatorcontrib><title>Morphological characterization of bovine primordial follicles and their environment in vivo</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 micromolar thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microgram), breadth (26.8 +/- 1.5 micrometer), and depth ( 30.4 +/- 1.4 micrometer) (mean +/- SEM; n</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Nucleus - ultrastructure</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>CHIMIE</subject><subject>Cytoplasm - ultrastructure</subject><subject>DIMENSION</subject><subject>ESTRUCTURA CELULAR</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa Cells - ultrastructure</subject><subject>IMMUNOLOGIE</subject><subject>INMUNOLOGIA</subject><subject>Mammalian female genital system</subject><subject>Microscopy, Electron</subject><subject>Morphology. Physiology</subject><subject>Oocytes - ultrastructure</subject><subject>OVAIRE</subject><subject>Ovarian Follicle - ultrastructure</subject><subject>OVARIOS</subject><subject>Ovary - blood supply</subject><subject>Ovary - cytology</subject><subject>QUIMICA</subject><subject>Stem Cells - ultrastructure</subject><subject>STRUCTURE CELLULAIRE</subject><subject>ULTRAESTRUCTURA</subject><subject>ULTRASTRUCTURE</subject><subject>VACA</subject><subject>VACHE</subject><subject>Vertebrates: reproduction</subject><subject>von Willebrand Factor - analysis</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpNkUtrHDEQhEVIsDdO_oCNQYeQ29h6jGakYzDOA2xycHzKQWg0rR0ZjbSWZnexf31kvASfGro-qukqhM4ouaBEicvBp5Bhk9MoxIWoO8LfoRUVTDU96-R7tCKEdA3nHT9GH0t5IIS2nPEjdCQVYYySFfp7m_JmSiGtvTUB28lkYxfI_tksPkWcHB7SzkfAm-znlEdfKZdC8DZAwSaOeJnAZwxx53OKM8QF-4h3fpc-oQ_OhAKfD_ME3X-__nP1s7n5_ePX1bebxjEplsYNYICMXAkqnLWuA-UsbS2zSloiu54MbeeYGnrWOiEFM5IpYWlHhQLiKD9BX199axSPWyiLnn2xEIKJkLZF91JQxqSs4PkB3A4zjPrlJZOf9CGNqn856KbUNFw20fryH-OsVT1_c2_y62nvM-gymxCqKdf7_V4ILfRLGRU8fQWdSdqsc_W6v1M97aSS_B-yq4eG</recordid><startdate>19961101</startdate><enddate>19961101</enddate><creator>Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.)</creator><creator>Rodgers, R.J</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19961101</creationdate><title>Morphological characterization of bovine primordial follicles and their environment in vivo</title><author>Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.) ; Rodgers, R.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f285t-fbeae0d39515fccf6e9fc14c2c98c08670b46f29b724f5852a8295c16159e0f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Nucleus - ultrastructure</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>CHIMIE</topic><topic>Cytoplasm - ultrastructure</topic><topic>DIMENSION</topic><topic>ESTRUCTURA CELULAR</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa Cells - ultrastructure</topic><topic>IMMUNOLOGIE</topic><topic>INMUNOLOGIA</topic><topic>Mammalian female genital system</topic><topic>Microscopy, Electron</topic><topic>Morphology. Physiology</topic><topic>Oocytes - ultrastructure</topic><topic>OVAIRE</topic><topic>Ovarian Follicle - ultrastructure</topic><topic>OVARIOS</topic><topic>Ovary - blood supply</topic><topic>Ovary - cytology</topic><topic>QUIMICA</topic><topic>Stem Cells - ultrastructure</topic><topic>STRUCTURE CELLULAIRE</topic><topic>ULTRAESTRUCTURA</topic><topic>ULTRASTRUCTURE</topic><topic>VACA</topic><topic>VACHE</topic><topic>Vertebrates: reproduction</topic><topic>von Willebrand Factor - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.)</creatorcontrib><creatorcontrib>Rodgers, R.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.)</au><au>Rodgers, R.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological characterization of bovine primordial follicles and their environment in vivo</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1996-11-01</date><risdate>1996</risdate><volume>55</volume><issue>5</issue><spage>1003</spage><epage>1011</epage><pages>1003-1011</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 micromolar thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microgram), breadth (26.8 +/- 1.5 micrometer), and depth ( 30.4 +/- 1.4 micrometer) (mean +/- SEM; n</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>8902210</pmid><doi>10.1095/biolreprod55.5.1003</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1996-11, Vol.55 (5), p.1003-1011 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Cattle Cell Nucleus - ultrastructure CELLULE CELULAS CHIMIE Cytoplasm - ultrastructure DIMENSION ESTRUCTURA CELULAR Female Fundamental and applied biological sciences. Psychology Granulosa Cells - ultrastructure IMMUNOLOGIE INMUNOLOGIA Mammalian female genital system Microscopy, Electron Morphology. Physiology Oocytes - ultrastructure OVAIRE Ovarian Follicle - ultrastructure OVARIOS Ovary - blood supply Ovary - cytology QUIMICA Stem Cells - ultrastructure STRUCTURE CELLULAIRE ULTRAESTRUCTURA ULTRASTRUCTURE VACA VACHE Vertebrates: reproduction von Willebrand Factor - analysis |
| title | Morphological characterization of bovine primordial follicles and their environment in vivo |
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