Chicken anti-protein A prevents Staphylococcus aureus protein A from binding to human and rabbit IgG in immunoassays and eliminates most false positive results

This report demonstrates that chicken anti-protein A can prevent both soluble and surface-bound Staphylococcal protein A from binding to either human or rabbit IgG. In an ELISA assay, chicken anti-protein A prevented > 98% of the soluble protein A from binding to the human IgG-Fc coat. In a blott...

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Bibliographic Details
Published in:Journal of immunological methods 1996-10, Vol.198 (1), p.67-77
Main Authors: Hoffman, Wayne L., Ruggles, Ann O., Tabarya, Daniel
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - immunology
Antibodies, Bacterial - metabolism
Binding, Competitive - immunology
Biological and medical sciences
Chicken anti-protein A
Chickens
ELISA
False Positive Reactions
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunoassay - methods
Immunoglobulin G - immunology
Immunoglobulin G - metabolism
Molecular immunology
Protein A
Protein Binding - immunology
Rabbits
Staphylococcal Protein A - immunology
Staphylococcal Protein A - metabolism
Staphylococcus aureus
Techniques
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Summary:This report demonstrates that chicken anti-protein A can prevent both soluble and surface-bound Staphylococcal protein A from binding to either human or rabbit IgG. In an ELISA assay, chicken anti-protein A prevented > 98% of the soluble protein A from binding to the human IgG-Fc coat. In a blotting assay, chicken anti-protein A prevented the membrane-bound protein A from interacting with the human IgG probe. When intact S. aureus (Cowan I strain) was bound to the surface of a microassay plate, chicken anti-protein A blocked > 98% of the cell wall protein A and permitted the probing of the surface components with human IgG. In another immunoassay, rabbit anti-enterotoxin A IgG was used to measure enterotoxin A concentrations in S. aureus culture medium supernatants after soluble protein A was blocked by chicken anti-protein A. Thus, the binding of chicken anti-protein A to protein A almost completely eliminates false positive results and permits the measurement of specific antibodies or antigens in a variety immunoassays where protein A is present.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(96)00152-4