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New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi
New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integ...
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| Published in: | Gene 1996-10, Vol.176 (1), p.93-96 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c478t-5ced9317ee43b8689df69d5e1066bc9eaec977760dce3ebf4eeb2f48d606fd7e3 |
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| cites | cdi_FETCH-LOGICAL-c478t-5ced9317ee43b8689df69d5e1066bc9eaec977760dce3ebf4eeb2f48d606fd7e3 |
| container_end_page | 96 |
| container_issue | 1 |
| container_start_page | 93 |
| container_title | Gene |
| container_volume | 176 |
| creator | An, Zhiqiang Farman, Mark L. Budde, Allen Taura, Satoru Leong, Sally A. |
| description | New cosmid vectors were constructed for the ascomycete fungus,
Magnaporthe grisea and the basidiomycete fungus,
Ustilago maydis. These vectors are capable of transforming
M. grisea at frequencies of up to 5 transformants/μg linear DNA and
U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable. |
| doi_str_mv | 10.1016/0378-1119(96)00225-9 |
| format | article |
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Magnaporthe grisea and the basidiomycete fungus,
Ustilago maydis. These vectors are capable of transforming
M. grisea at frequencies of up to 5 transformants/μg linear DNA and
U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(96)00225-9</identifier><identifier>PMID: 8918238</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Ascomycetes ; Ascomycota - genetics ; Base Sequence ; Basidiomycetes ; Chromosome Walking ; construction ; Cosmids ; DNA, Bacterial ; genbank/l76723 ; Gene Library ; genetic markers ; genetic transformation ; Genetic Vectors ; Magnaporthe grisea ; Molecular Sequence Data ; nucleotide sequences ; Promoter ; restriction enzyme mediated integration ; Restriction Mapping ; Transformant ; Transformation, Genetic ; Ustilago - genetics ; Ustilago maydis ; Ustilago zeae</subject><ispartof>Gene, 1996-10, Vol.176 (1), p.93-96</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-5ced9317ee43b8689df69d5e1066bc9eaec977760dce3ebf4eeb2f48d606fd7e3</citedby><cites>FETCH-LOGICAL-c478t-5ced9317ee43b8689df69d5e1066bc9eaec977760dce3ebf4eeb2f48d606fd7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8918238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>An, Zhiqiang</creatorcontrib><creatorcontrib>Farman, Mark L.</creatorcontrib><creatorcontrib>Budde, Allen</creatorcontrib><creatorcontrib>Taura, Satoru</creatorcontrib><creatorcontrib>Leong, Sally A.</creatorcontrib><title>New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi</title><title>Gene</title><addtitle>Gene</addtitle><description>New cosmid vectors were constructed for the ascomycete fungus,
Magnaporthe grisea and the basidiomycete fungus,
Ustilago maydis. These vectors are capable of transforming
M. grisea at frequencies of up to 5 transformants/μg linear DNA and
U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.</description><subject>Ascomycetes</subject><subject>Ascomycota - genetics</subject><subject>Base Sequence</subject><subject>Basidiomycetes</subject><subject>Chromosome Walking</subject><subject>construction</subject><subject>Cosmids</subject><subject>DNA, Bacterial</subject><subject>genbank/l76723</subject><subject>Gene Library</subject><subject>genetic markers</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>Magnaporthe grisea</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Promoter</subject><subject>restriction enzyme mediated integration</subject><subject>Restriction Mapping</subject><subject>Transformant</subject><subject>Transformation, Genetic</subject><subject>Ustilago - genetics</subject><subject>Ustilago maydis</subject><subject>Ustilago zeae</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkUuPFCEUhYnRjO3oP9DIymhiKRTFa2NiJr6SiS501oSCS4tWQQtVM_HfS9mdWSobAue7JyfnIvSYkleUUPGaMKk6Sql-rsULQvqed_oO2lEldUcIU3fR7ha5jx7U-oO0w3l_hs6Upqpnaoemz3CDXa5z9Pga3JJLxSEXPMWx2PK7SakuZXVLzOkldt9LnnPNM-AbO_2MaY9t8rhAY-JfBs_2cNj-Y8IhTnaGtOS1ea5pHx-ie8FOFR6d7nN09f7dt4uP3eWXD58u3l52bpBq6bgDrxmVAAMblVDaB6E9B0qEGJ0GC05LKQXxDhiMYQAY-zAoL4gIXgI7R8-OvoeSf60tnJljdTBNNkELY6TiTGki_wtSrogUnDRwOIKu5FoLBHMocW4FGUrMtg2zVW22qo1uj20bRrexJyf_dZzB3w6d6m_606MebDZ2X2I1V197QhmhvB_0sDm8ORLQ-rqOUEx1EVJrKJa2LuNz_HeEPyOgpW4</recordid><startdate>19961017</startdate><enddate>19961017</enddate><creator>An, Zhiqiang</creator><creator>Farman, Mark L.</creator><creator>Budde, Allen</creator><creator>Taura, Satoru</creator><creator>Leong, Sally A.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19961017</creationdate><title>New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi</title><author>An, Zhiqiang ; Farman, Mark L. ; Budde, Allen ; Taura, Satoru ; Leong, Sally A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-5ced9317ee43b8689df69d5e1066bc9eaec977760dce3ebf4eeb2f48d606fd7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Ascomycetes</topic><topic>Ascomycota - genetics</topic><topic>Base Sequence</topic><topic>Basidiomycetes</topic><topic>Chromosome Walking</topic><topic>construction</topic><topic>Cosmids</topic><topic>DNA, Bacterial</topic><topic>genbank/l76723</topic><topic>Gene Library</topic><topic>genetic markers</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>Magnaporthe grisea</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Promoter</topic><topic>restriction enzyme mediated integration</topic><topic>Restriction Mapping</topic><topic>Transformant</topic><topic>Transformation, Genetic</topic><topic>Ustilago - genetics</topic><topic>Ustilago maydis</topic><topic>Ustilago zeae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>An, Zhiqiang</creatorcontrib><creatorcontrib>Farman, Mark L.</creatorcontrib><creatorcontrib>Budde, Allen</creatorcontrib><creatorcontrib>Taura, Satoru</creatorcontrib><creatorcontrib>Leong, Sally A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>An, Zhiqiang</au><au>Farman, Mark L.</au><au>Budde, Allen</au><au>Taura, Satoru</au><au>Leong, Sally A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-10-17</date><risdate>1996</risdate><volume>176</volume><issue>1</issue><spage>93</spage><epage>96</epage><pages>93-96</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>New cosmid vectors were constructed for the ascomycete fungus,
Magnaporthe grisea and the basidiomycete fungus,
Ustilago maydis. These vectors are capable of transforming
M. grisea at frequencies of up to 5 transformants/μg linear DNA and
U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8918238</pmid><doi>10.1016/0378-1119(96)00225-9</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1996-10, Vol.176 (1), p.93-96 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78538907 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Ascomycetes Ascomycota - genetics Base Sequence Basidiomycetes Chromosome Walking construction Cosmids DNA, Bacterial genbank/l76723 Gene Library genetic markers genetic transformation Genetic Vectors Magnaporthe grisea Molecular Sequence Data nucleotide sequences Promoter restriction enzyme mediated integration Restriction Mapping Transformant Transformation, Genetic Ustilago - genetics Ustilago maydis Ustilago zeae |
| title | New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi |
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