Bombyx and Manduca prothoracicotropic hormones: an immunologic test for relatedness

Prothoracicotropic hormone (PTTH) controls the synthesis of ecdysteroids (molting hormones) by the insect prothoracic gland and thereby plays a critical role in insect growth, molting, and metamorphosis. PTTH has been purified, and cDNA clones obtained, from only one insect, the silkmoth Bombyx mori...

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Bibliographic Details
Published in:General and comparative endocrinology 1996-05, Vol.102 (2), p.247-254
Main Authors: Rybczynski, R, Mizoguchi, A, Gilbert, L I
Format: Article
Language:English
Subjects:
Adsorption
animal glands
Animals
Antibodies, Monoclonal - chemistry
anticorps monoclonal
anticuerpos monoclonales
Bombyx - metabolism
bombyx mori
chemistry
chimie
Cloning, Molecular
Ecdysterone - biosynthesis
Endocrine Glands - drug effects
Endocrine Glands - metabolism
Endocrine Glands - physiology
evolucion
evolution
glande animale
glandulas animales
hormonas
hormonas de la muda
hormone
hormone de mue
hormones
Immunoblotting
immunologie
immunology
inmunologia
Insect Hormones - biosynthesis
Insect Hormones - chemistry
Insect Hormones - pharmacology
Manduca - metabolism
manduca sexta
metabolisme des steroides
metabolismo de esteroides
monoclonal antibodies
moulting hormones
Protein Biosynthesis
protein synthesis
quimica
Radioimmunoassay
sintesis de proteinas
steroid metabolism
Stimulation, Chemical
synthese proteique
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Summary:Prothoracicotropic hormone (PTTH) controls the synthesis of ecdysteroids (molting hormones) by the insect prothoracic gland and thereby plays a critical role in insect growth, molting, and metamorphosis. PTTH has been purified, and cDNA clones obtained, from only one insect, the silkmoth Bombyx mori. Recently, a partial amino acid sequence was obtained for a putative PTTH from the tobacco hornworm, Manduca sexta, and the data suggested an unexpected homology to a vertebrate retinoid binding protein rather than to the Bombyx PTTH. In this study, a monoclonal antibody against the Bombyx PTTH was added to in vitro assays that assess the ability of partially purified Manduca PTTH to stimulate prothoracic gland ecdysteroid and protein synthesis. The results indicate that Bombyx and Manduca PTTHs are indeed members of the same protein family that have likely diverged in the PTTH receptor-binding region.
ISSN:0016-6480
1095-6840
DOI:10.1006/gcen.1996.0066