Biological variation of lipids and lipoproteins in fingerstick blood

The biological variability of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and calculated low-density lipoprotein cholesterol (LDL-C) was determined in three serial (monthly) capillary and venous specimens from 83 subjects. The analytes were quantified wi...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1996-12, Vol.42 (12), p.2002-2007
Main Authors: Kafonek, SD, Donovan, L, Lovejoy, KL, Bachorik, PS
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Capillaries
Cholesterol - blood
Cholesterol, HDL - blood
Cholesterol, LDL - blood
Female
Fingers - blood supply
Humans
Investigative techniques, diagnostic techniques (general aspects)
Lipids - blood
Lipoproteins - blood
Male
Medical sciences
Metabolic diseases
Middle Aged
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Point-of-Care Systems
Quality Control
Veins
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Summary:The biological variability of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and calculated low-density lipoprotein cholesterol (LDL-C) was determined in three serial (monthly) capillary and venous specimens from 83 subjects. The analytes were quantified with a desktop analyzer. We saw no differences in the coefficient of biological variability (CVb) between capillary and venous specimens for any analyte (TC, 5.2%; TG, 14.7%; HDL-C, 7.2%; LDL-C, 5.4%). The average analytical variability (CVa) for each analyte, determined in quality-control pools, was; TC, 5.0%; TG, 5.2%; HDL-C, 5.8%; and LDL-C, 7.5%. Compared with standardized laboratory measurements, the desktop analyzer exhibited a significant (P < 0.001) positive bias for all analytes (average bias: TC, 5%; TG, 16%; HDL-C, 6%; and LDL-C, 2.4%). Thus, the biological variation of lipids and lipoproteins was the same in fingerstick and venous samples, and the desktop analyzer showed inherently greater analytical variability.
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/42.12.2002