Mechanical strain-induced proliferation of osteoblastic cells parallels increased TGF-beta 1 mRNA

It is well known that mechanical stimulation can prompt healing of bone fractures. However, the mechanism involved is less clear. In this study, we found that a 0.17% cyclic, biaxial mechanical strain delivered at 1 Hz increased proliferation of MC3T3-E1 cells, a clonal osteoblastic cell line. Mecha...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-12, Vol.229 (2), p.449-453
Main Authors: Zhuang, H, Wang, W, Tahernia, A D, Levitz, C L, Luchetti, W T, Brighton, C T
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Calmodulin - metabolism
Cell Division - genetics
Inositol Phosphates - metabolism
Mice
Osteoblasts - cytology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transforming Growth Factor beta - genetics
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Summary:It is well known that mechanical stimulation can prompt healing of bone fractures. However, the mechanism involved is less clear. In this study, we found that a 0.17% cyclic, biaxial mechanical strain delivered at 1 Hz increased proliferation of MC3T3-E1 cells, a clonal osteoblastic cell line. Mechanical strain also increased the level of TGF-beta 1 mRNA determined by quantitative reverse transcription/ polymerase chain reaction. Previous reports have shown that neomycin and W-7, which are inhibitors in the inositol phosphate/calmodulin pathway, blocked mechanical strain-induced proliferation of the osteoblast cells. Interestingly, we found that neomycin and W-7 can also block mechanical stimulation-induced elevation of TGF-beta 1 mRNA. Finally, using an antibody which blocked the action of TGF-beta 1, we found that the increased MC3T3-E1 cell proliferation induced by mechanical strain did not depend on the action of TGF-beta 1.
ISSN:0006-291X
DOI:10.1006/bbrc.1996.1824