A Novel Member of the RING Finger Family, KRIP-1, Associates with the KRAB-A Transcriptional Repressor Domain of Zinc Finger Proteins

The Kruppel-associated box A (KRAB-A) domain is an evolutionarily conserved transcriptional repressor domain present in approximately one-third of zinc finger proteins of the Cys2-His2type. Using the yeast two-hybrid system, we report the isolation of a cDNA encoding a novel murine protein, KRAB-A i...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-12, Vol.93 (26), p.15299-15304
Main Authors: Kim, Sung-Su, Chen, Yung-Ming, O'Leary, Eileen, Witzgall, Ralph, Vidal, Marc, Bonventre, Joseph V.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Antibodies
Base Sequence
Biological Sciences
Complementary DNA
Conserved Sequence
COS Cells
Deoxyribonucleic acid
DNA
Embryo, Mammalian
Gene Library
Genetics
Kidney - metabolism
Mice
Molecular Sequence Data
Nuclear Proteins - biosynthesis
Nuclear Proteins - chemistry
Nuclear Proteins - metabolism
Plasmids
Proteins
Recombinant Fusion Proteins - metabolism
Repression
Repressor Proteins - metabolism
Transcription Factors - biosynthesis
Transcription Factors - chemistry
Transcription Factors - metabolism
Transfection
Tripartite Motif-Containing Protein 28
Yeast
Yeasts
Zinc
Zinc Fingers
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Summary:The Kruppel-associated box A (KRAB-A) domain is an evolutionarily conserved transcriptional repressor domain present in approximately one-third of zinc finger proteins of the Cys2-His2type. Using the yeast two-hybrid system, we report the isolation of a cDNA encoding a novel murine protein, KRAB-A interacting protein 1 (KRIP-1) that physically interacts with the KRAB-A region. KRIP-1 is a member of the RBCC subfamily of the RING finger, or Cys3HisCys4, family of zinc binding proteins whose other members are known to play important roles in differentiation, oncogenesis, and signal transduction. The KRIP-1 protein has high homology to TIF1, a putative modulator of ligand-dependent activation function of nuclear receptors. A 3.5-kb mRNA for KRIP-1 is ubiquitously expressed among all adult mouse tissues studied. When a GAL4-KRIP-1 fusion protein is expressed in COS cells with a chloramphenicol acetyltransferase reporter construct with five GAL4 binding sites, there is dose-dependent repression of transcription. Thus, KRIP-1 interacts with the KRAB-A region of C2H2zinc finger proteins and may mediate or modulate KRAB-A transcriptional repressor activity.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.26.15299