Synthetic peptides entrapped in microparticles can elicit cytotoxic T cell activity

• Published in: Vaccine 1996-11, Vol.14 (16), p.1523-1530
• Main Authors: Nixon, Douglas F., Hioe, Catarina, Chen, Pei-de, Bian, Zuning, Kuebler, Peter, Li, Ming-Lie, Qiu, Howard, Li, Xuan-Mao, Singh, Manmohan, Richardson, Julie, McGee, Paul, Zamb, Tim, Koff, Wayne, Wang, Chang Yi, O'Hagan, Derek
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Antigens, Protozoan - biosynthesis; Antigens, Protozoan - immunology; Antigens, Viral - biosynthesis; Antigens, Viral - immunology; Applied microbiology; Biological and medical sciences; CTL; Emulsions; Female; Fundamental and applied biological sciences. Psychology; Glycine max - immunology; Influenza A virus - immunology; Lactic Acid; lipopeptides; Lipoproteins - immunology; Mice; Mice, Inbred BALB C; Microbiology; Microparticles; Microspheres; peptides; Peptides - chemical synthesis; Peptides - immunology; Plasmodium berghei; Plasmodium berghei - immunology; Polyglycolic Acid; Polylactic Acid-Polyglycolic Acid Copolymer; Polymers - therapeutic use; T-Lymphocytes, Cytotoxic - immunology; vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Virology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(96)00099-0

Peptides from Plasmodium berghei circumsporozoite protein (CS) and influenza A virus nucleoprotein (NP) were entrapped in microparticles prepared from poly (lactide-co-glycolide) polymers, and the microparticles were administered parenterally to mice. After immunization with single or multiple doses, splenocytes were tested for a cytotoxic T cell (CTL) response and high levels of CTL activity were detected. The CTL induced were CD8 +, MHC class I restricted, and could recognize virus infected cells. Peptide entrapped in microparticles of mean size 2 μm and above). Microparticles could also be used to deliver lipid modified peptides (lipopeptides) and elicited higher levels of cytolytic activity than either free peptide in microparticles or lipopeptide alone. Microparticles provide a novel way of inducing a CTL response using synthetic peptides.