Bovine vitamin A and beta-carotene intake and lactational status. 2. Responsiveness of mitogen-stimulated peripheral blood lymphocytes to vitamin A and beta-carotene challenge in vitro

The interaction of dietary vitamin A and beta-carotene with lactational status on the in vitro proliferation of mitogen-induced peripheral blood lymphocytes was studied. Cows were fed (IU/cow per d) 1) 53,000 IU vitamin A, 2) 213,000 IU vitamin A, or 3) 53,000 IU vitamin A plus 400 mg beta-carotene...

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Bibliographic Details
Published in:Journal of dairy science 1988-11, Vol.71 (11), p.3120-3127
Main Authors: Tjoelker, L.W, Chew, B.P, Tanaka, T.S, Daniel, L.R
Format: Article
Language:English
Subjects:
ABSORCION DE SUBSTANCIAS NUTRITIVAS
ABSORPTION DE SUBSTANCES NUTRITIVES
Animals
beta Carotene
BOVIN LAITIER
CAROTENOIDE
CAROTENOIDES
CAROTENOIDS
Carotenoids - pharmacology
Cattle - blood
Concanavalin A - pharmacology
COWS
DAIRY CATTLE
DRY PERIOD
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
Female
GANADO DE LECHE
IN VITRO EXPERIMENTATION
LACTACION
LACTATION
Lactation - blood
Lactation - physiology
LINFOCITOS
Lipopolysaccharides - pharmacology
LYMPHOCYTE
LYMPHOCYTES
Lymphocytes - drug effects
Lymphocytes - physiology
Mitogens - pharmacology
NUTRIENT UPTAKE
PERIODE DE TARISSEMENT
PERIODO DE SECADO
Pregnancy
RETINOL
VACA
VACHE
Vitamin A - pharmacology
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Summary:The interaction of dietary vitamin A and beta-carotene with lactational status on the in vitro proliferation of mitogen-induced peripheral blood lymphocytes was studied. Cows were fed (IU/cow per d) 1) 53,000 IU vitamin A, 2) 213,000 IU vitamin A, or 3) 53,000 IU vitamin A plus 400 mg beta-carotene from 6 wk before to 2 wk after dry off. Lymphocytes were incubated with retinol, retinoic acid, or beta-carotene. Concanavalin A-induced blastogenesis was inhibited by 10(-6) M retinol and 10(-8) M retinoic acid in cows fed 53,000 IU vitamin A before dry off. In contrast, 10(-7) M retinol and 10(-7) M retinoic acid stimulated Concanavalin A-induced blastogenesis for cows fed vitamin A plus beta-carotene before dry off. After dry off, retinol and retinoic acid did not affect Concanavalin A-induced blastogenesis in all treatment groups. In vitro, 10(-5) M beta-carotene inhibited Concanavalin A-induced blastogenesis before and after dry off in all treatment groups. Blastogenesis in the absence of mitogen stimulation or induced by lipopolysaccharide was inhibited by all vitamins before and after dry off in all treatment groups. These data indicate that vitamin A and beta-carotene supplementation interact with lactational status to influence the responsiveness of bovine blood lymphocytes to vitamin challenge in vitro.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(88)79912-9