Hepatitis B virus genotyping: current methods and clinical implications

Summary Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma (HCC). Eight different HBV genotypes have been identified with distinct geographical distributions. Different genotyping methods exist including sequencing, INNO-LiPA, restriction fragment polymorphism (...

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Bibliographic Details
Published in:International journal of infectious diseases 2010-11, Vol.14 (11), p.e941-e953
Main Authors: Guirgis, Bassem S.S, Abbas, Radwa O, Azzazy, Hassan M.E
Format: Article
Language:English
Subjects:
Antiviral Agents - therapeutic use
Carcinoma, Hepatocellular - genetics
Disease Transmission, Infectious
DNA, Viral - analysis
DNA, Viral - genetics
Genome, Viral
Genotype
Genotyping methods
HBV genotype
HBV transmission
Hepatitis B virus - classification
Hepatitis B virus - genetics
Hepatitis B, Chronic - therapy
Hepatitis B, Chronic - transmission
Hepatitis B, Chronic - virology
Humans
Infectious Disease
Liver Cirrhosis - virology
Liver Neoplasms - virology
Liver Transplantation
Polymorphism, Restriction Fragment Length
Prognosis
Pulmonary/Respiratory
Therapeutic response
Transplantation outcome
Treatment Outcome
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Summary:Summary Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma (HCC). Eight different HBV genotypes have been identified with distinct geographical distributions. Different genotyping methods exist including sequencing, INNO-LiPA, restriction fragment polymorphism (RFLP), multiplex PCR, serotyping, oligonucleotide microarray chips, reverse dot blot, restriction fragment mass polymorphism (RFMP), invader assay, and real-time PCR. Several investigators have studied the influence of HBV genotypes on clinical outcomes in chronic HBV patients. This review describes the current genotyping techniques, as well as their advantages and limitations. It also presents the clinical evidence that correlates HBV genotypes to clinical outcomes including disease severity, HCC development, response to therapy, disease chronicity, transplantation outcomes, and occult infection.
ISSN:1201-9712
1878-3511
DOI:10.1016/j.ijid.2010.03.020