Clearance rates of biotinylated ferritins in canine: a possible physiological role of canine serum ferritin as an iron transporter

To elucidate the physiological role of canine serum ferritin, we measured clearance rates of biotinylated ferritins in beagle. Biotinylated canine tissue ferritins were cleared rapidly from circulation. The clearance time (T 1/2) of liver ferritin (H/L subunit ratio = 0.43) was 6.8 to 11.8 min, and...

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Bibliographic Details
Published in:Biometals 2003-06, Vol.16 (2), p.341-347
Main Authors: Orino, Koichi, Terada, Ayumi, Yoshida, Akiko, Okano, Shozo, Higuchi, Seiichi, Natsuhori, Masahiro, Watanabe, Kiyotaka
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Biotinylation
Body Weight
Circulation
Clearances
Dogs
Electrophoresis, Polyacrylamide Gel
Female
Ferritin
Ferritins - blood
Ferritins - metabolism
Ferritins - pharmacokinetics
Heart
Iron
Iron - metabolism
Liver
Liver - metabolism
Male
Metabolic Clearance Rate
Physiology
Recombinant
Serums
Transporter
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Summary:To elucidate the physiological role of canine serum ferritin, we measured clearance rates of biotinylated ferritins in beagle. Biotinylated canine tissue ferritins were cleared rapidly from circulation. The clearance time (T 1/2) of liver ferritin (H/L subunit ratio = 0.43) was 6.8 to 11.8 min, and that of heart ferritin (H/L = 3.69) was 9.3 to 25.0 min. T 1/2 of biotinylated canine liver ferritin was independentof iron content, whereas canine heart apoferritin (T 1/2 = 31.2 and 32.7 min) was more slowly removed from circulation than the holoferritin. On the other hand, biotinylated recombinant bovine H-chain ferritin homopolymer show a much slower rate of removal (T 1/2 = 153.8 and 155.0 min) compared with the L-chain ferritin homopolymer (T 1/2 = 26.4 and 31.3 min). The rapid clearance of canine tissue ferritin suggests that serum ferritin is an iron transporter in canines.
ISSN:0966-0844
1572-8773
DOI:10.1023/A:1020659317564