Use of purified porcine follicle-stimulating hormone for ovarian stimulation of macaque monkeys

At present, in nonhuman primates, ovarian stimulation with heterologous gonadotropin preparations is the only reliable way to produce substantial numbers of competent ova for in vitro fertilization and embryo development studies. Preparations such as equine chorionic gonadotropin (eCG) and human men...

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Bibliographic Details
Published in:Theriogenology 1996-03, Vol.45 (4), p.727-732
Main Authors: Schramm, R.D., Bavister, B.D.
Format: Article
Language:English
Subjects:
blastocyst development
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
EMBRIONES ANIMALES
EMBRYON ANIMAL
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
FECONDATION
FECUNDACION
FSH
GONADOTROPHINE
GONADOTROPINAS
HFS
in vitro fertilization
MEIOSE
MEIOSIS
MONO
ovarian stimulation
OVULE
OVULO
porcine FSH
rhesus monkey
SINGE
SUPEROVULACION
SUPEROVULATION
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Summary:At present, in nonhuman primates, ovarian stimulation with heterologous gonadotropin preparations is the only reliable way to produce substantial numbers of competent ova for in vitro fertilization and embryo development studies. Preparations such as equine chorionic gonadotropin (eCG) and human menopausal gonadotropins (hMG or hFSH) have been used successfully, but eCG is crude and contains variable amounts of LH activity, while hMG/hFSH is very expensive and the supply is not stable. This study examined the use of a purified porcine FSH preparation (Folltropin V) for ovarian stimulation in rhesus monkeys. Twice-daily intramuscular injections of this preparation resulted in good follicular development, and was followed by a single intramuscular injection of hCG. Ova were collected laparoscopically 30 h post hCG, fertilized in vitro and then cultured until development ceased. Stimulation of 9 monkeys with Folltropin V yielded a mean of 20 ova per animal, of which 71% reached metaphase II and were inseminated; of these, 92% were fertilized in vitro and 48% developed into blastocysts in vitro. These results are similar to those reported by us and by others using eCG, hMG or an hFSH/hMG combination for ovarian stimulation of macaque monkeys. We conclude that Folltropin V is a suitable alternative preparation for ovarian stimulation in nonhuman primates and one that also has the advantages of being readily available and much less expensive than human gonadotropin preparations.
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(96)00002-7