Stainless Steel Mesh Supports High Density Cell Growth and Production of Recombinant Mullerian Inhibiting Substances

• Published in: In Vitro Cellular & Developmental Biology 1989-02, Vol.25 (2), p.213-216
• Main Authors: Epstein, James, Desjardins, Edward J., Hudson, Peter L., Donahoe, Patricia K.
• Format: Article
• Language: English
• Subjects: Animal cells; Animals; Anti-Mullerian Hormone; Biological and medical sciences; Biotechnology; Cell Adhesion; cell culture; Cell culture techniques; Cell cultures. Hybridization. Fusion; Cell Division; Cell growth; Cell Line - cytology; Cell lines; CHO cells; Collagen - pharmacology; Cultured cells; Doubling time; Eukaryotic cell cultures; Extracellular Matrix; Flasks; Fundamental and applied biological sciences. Psychology; Glycoproteins; Growth Inhibitors; immobilized cells; Membrane Glycoproteins - pharmacology; Methods. Procedures. Technologies; Miscellaneous; Molecular and cellular biology; Molecular Biology - instrumentation; Mullerian Inhibiting Substance; proteins; Rapid Communications in Cell Biology; Recombinant proteins; Recombinant Proteins - biosynthesis; recombinants; Stainless Steel; Stainless steels; Surface areas; Surface Properties; Testicular Hormones - biosynthesis
• ISSN: 0883-8364; 0073-5655; 2327-431X; 1475-2689
• DOI: 10.1007/BF02626181

Stainless steel mesh supported the high density growth of anchorage dependent CHO fibroblasts without the use of a special culture system. CHO cells, designated B-9, containing an amplified genomic construct of the human gene for Mullerian Inhibiting Substance (MIS), grew to a high confluent density on stainless steel meshwork while producing substantial amounts of human recombinant MIS over a long period of time. The mesh could be easily coated with various extracellular matrix proteins, such as Laminin, Fibronectin, Collagen or Matrigel, which permitted the testing of the effects of surface modifications on cell yield and recombinant protein production. Since the amount of medium per surface area required for optimal cell growth is lower than for some large volume cell culture methods, media costs can be reduced using mesh. In addition, no special cell culture equipment or complex manipulations are required. Thus, the use of meshwork for anchorage-dependent cells can increase the efficiency of growth and decrease the cost of recombinant protein production.