Mononuclear cells from human lung parenchyma support antigen-induced T lymphocyte proliferation

We have previously demonstrated that there is a subpopulation of loosely adherent pulmonary mononuclear cells that can be isolated from minced and enzyme‐digested lung tissue with a potent capacity to stimulate allogeneic T lymphocyte proliferation. We now demonstrate that these cells are also capab...

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Bibliographic Details
Published in:Journal of leukocyte biology 1989-04, Vol.45 (4), p.336-344
Main Authors: Nicod, Laurent P., Lipscomb, Mary F., Weissler, Jonathan C., Toews, Galen B.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
antigen presentation
Antigens, Viral - immunology
Biological and medical sciences
Bronchoalveolar Lavage Fluid - immunology
Cell Adhesion
Cell Separation
Fundamental and applied biological sciences. Psychology
Fundamental immunology
human lung
Humans
Immunobiology
Leukocytes, Mononuclear - classification
Leukocytes, Mononuclear - immunology
Lung - immunology
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Macrophages - classification
Macrophages - metabolism
Monocytes - immunology
Organs and cells involved in the immune response
Phagocytes
Phenotype
pulmonary interstitium
Receptors, Fc
T-Lymphocytes - immunology
Tetanus Toxoid - immunology
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Summary:We have previously demonstrated that there is a subpopulation of loosely adherent pulmonary mononuclear cells that can be isolated from minced and enzyme‐digested lung tissue with a potent capacity to stimulate allogeneic T lymphocyte proliferation. We now demonstrate that these cells are also capable of stimulating an autologous mixed leukocyte reaction (AMLR) and presenting antigen to autologous T lymphocytes. These loosely adherent mononuclear cells (LAM) were more effective than either alveolar macrophages or monocytes as antigen‐presenting cells. Depletion of phagocytic or Fc receptor‐positive cells from the LAM population enhanced the stimulation of an reaction AMLR while preserving antigen‐induced T lymphocyte proliferation. These results indicate that there are nonphagocytic, Fc receptor‐negative accessory cells in human lung parenchyma capable of activating resting T cells in an AMLR and supporting antigen‐specific T lymphocyte proliferation. The identity of these cells is uncertain, but the data strongly suggest that the cell is not a classical monocyte‐derived macrophage. These antigen‐presenting cells may be critical in the initiation of immune responses within the lung.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.45.4.336