Coating in vitro of erythrocytes with Tamm Horsfall-Protein

A method is described to coat isolated peripheral blood erythrocytes in vitro with Tamm-Horsfall Protein (THP, uromodulin). Coating of erythrocytes with THP was accomplished by incubation of the cells in the presence of THP, made monomeric by incubation in a high urea concentration. THP-coating of e...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 1997-02, Vol.258 (2), p.179-192
Main Authors: Janssens, Pim M.W., Kuiper, Sandra, de Vries, H., Willems, Johannes L., Klasen, Ina, van Oosterwijk, Monique, Paardekooper, Jolanda
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - blood
Blood Preservation
Cell Separation
Erythrocytes
Erythrocytes - metabolism
Humans
In vitro coating
Mucoproteins - blood
Mucoproteins - metabolism
Mucoproteins - urine
Polymers
Protein Binding
Tamm Horsfall protein
Uromodulin
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method is described to coat isolated peripheral blood erythrocytes in vitro with Tamm-Horsfall Protein (THP, uromodulin). Coating of erythrocytes with THP was accomplished by incubation of the cells in the presence of THP, made monomeric by incubation in a high urea concentration. THP-coating of erythrocytes was dependent on the THP-concentration, maximal coating being obtained at a protein concentration _ ̆ 250 mg/ml . The best coating results were obtained if, during the co-incubation of erythrocytes with THP, urea was removed while the sodium chloride concentration was increased up to a physiologic concentration by means of dialysis. This alteration in chemical conditions promotes THPpolymerisation. Erythrocytes coated in this way could be preserved for at least 5 weeks in preserving solution, making them an interesting source of testing and control material. Coating of erythrocytes with THP could also be accomplished under conditions in which THP was preserved in a monomeric form, which suggests that peripheral blood erythrocytes have binding-sites for THP.
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(96)06452-2