Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors

• Published in: Gene 1997-03, Vol.187 (2), p.259-266
• Main Authors: Mita, Kazuei, Morimyo, Mitsuoki, Ito, Koichi, Sugaya, Kimihiko, Ebihara, Kanae, Hongo, Etsuko, Higashi, Tomoyasu, Hirayama, Yasuko, Nakamura, Yoshikazu
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; amino-acyl transfer rna; Animals; Base Sequence; binding proteins; cDNA catalog; chromosome mapping; Cloning, Molecular; complementary DNA; DNA, Fungal; Fission yeast; Fungal Proteins; genbank/d82571; genbank/d82572; genbank/d82573; genbank/d82574; genbank/d82575; gene expression; Genes, Fungal; heat stress; Humans; messenger RNA; Molecular Sequence Data; Nucleotide sequence; nucleotide sequences; nutrient deficiencies; Peptide Elongation Factor 1; Peptide Elongation Factor 2; Peptide Elongation Factors - genetics; Protein Biosynthesis; Saccharomyces cerevisiae Proteins; Schizosaccharomyces - genetics; Schizosaccharomyces pombe; Sequence Homology, Amino Acid; structural genes; transfer RNA; translation; Translation elongation factor; translation factors; ultraviolet radiation
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(96)00764-0

In the course of the Schizosaccharomyces pombe cDNA project, we succeeded in cloning all the genes encoding translation elongation factors EF-1α, EF-1β, EF-1γ, EF-2 and EF-3. With the exception of the EF-1γ gene, the nucleotide (nt) sequence of S. pombe elongation factors has not been previously reported. For EF-1α, we found three genes whose amino acid (aa) sequences are quite homologous each other (99.5%), but whose 3′ untranslated regions (UTRs) are completely different. Southern blot indicated that those three EF-1α genes are located at different loci. Northern analysis indicated that one of three EF-1α genes was inducible with UV-irradiation, while the level of expression for another of three EF-1α genes was repressed by UV and heat-shock (HS) treatments. The aa sequence predicted from the nt sequence of the S. pombe EF-1β cDNA clone covered almost all the coding sequence (CDS) of EF-1β except the first methionine which has 55.4% identity with that of S. cerevisiae. We also identified two copies of S. pombe EF-2 genes. Their aa sequences deduced from nt sequences are identical (100%), but they have different 3′ UTRs. The location of these two EF-2 genes in different loci was proved by Southern analysis. The S. pombe EF-3 cDNA clone encoded only a third of the CDS from the C-terminal and its deduced aa sequence has a 76% identity with those of other yeasts and fungi.