Fertilization-promoting peptide in reproductive tissues and semen of the male marmoset (Callithrix jacchus)

• Published in: Molecular reproduction and development 1997-05, Vol.47 (1), p.113-119
• Main Authors: Kennedy, Ann M., Morrell, Jane M., Siviter, Richard J., Cockle, Sheena M.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Bulbourethral Glands - chemistry; Callithrix - metabolism; Castration; Chromatography, Ion Exchange; Fundamental and applied biological sciences. Psychology; Male; male fertility; Mammalian male genital system; Morphology. Physiology; prostate; Prostate - chemistry; Pyrrolidonecarboxylic Acid - analogs & derivatives; Radioimmunoassay; Semen - chemistry; Semen - enzymology; Serine Endopeptidases - metabolism; Testis - chemistry; Testosterone - blood; thyrotrophin-releasing hormone; Thyrotropin-Releasing Hormone - analogs & derivatives; Thyrotropin-Releasing Hormone - analysis; Thyrotropin-Releasing Hormone - isolation & purification; Thyrotropin-Releasing Hormone - therapeutic use; Vertebrates: reproduction
• ISSN: 1040-452X; 1098-2795
• DOI: 10.1002/(SICI)1098-2795(199705)47:1<113::AID-MRD15>3.0.CO;2-2

Fertilization‐promoting peptide (FPP) is present in the prostate gland and semen of some mammals, and has been shown to enhance the fertilizing ability of both epididymal mouse and ejaculated human spermatozoa. The novel peptide may prove of importance for the treatment of some cases of male infertility, and a suitable animal model would be useful to test this hypothesis. To this end, we examined reproductive tissues and semen of the male marmoset for the presence of FPP. Peptides were extracted from seminal plasma, testes, prostate, and bulbourethral glands of intact and castrated male marmosets. The peptides were identified by ion‐exchange chromatography followed by radioimmunoassay. The mean concentration of FPP immunoreactivity in semen from intact males was 58.7 nM (SE ± 9.9 nM, n = 10), and anion‐exchange chromatography revealed FPP as the only immunoreactive peptide present. Analysis of tissues revealed that FPP in semen was likely to be derived from the prostate gland, which contained this peptide as the major source of immunoreactivity (10.86 pmol/gland; SE ± 4.39 pmol/gland, n = 4). Only low concentrations of FPP were detectable in the bulbourethral glands, and the peptide was undetectable in the testis. Surprisingly, FPP was readily detectable in the seminal plasma from one castrated marmoset and was present in the prostate gland from 3 castrates at levels which did not differ significantly from those in intact animals (5.47 pmol/gland, SE ± 1.64 pmol/gland, n = 3). Plasma testosterone measurements indicated that residual circulatory androgens remained after castration, which may be consistent both with the maintenance of mating behavior and the presence of prostatic FPP. We conclude that FPP is present within the prostate gland and seminal plasma of the marmoset at concentrations consistent with a role in male fertility in this species. Mol. Reprod. Dev. 47:113–119, 1997. © 1997 Wiley‐Liss, Inc.