Enhancement of the Mitogenic Effect by Artificial Juxtacrine Stimulation Using Immobilized EGF

Mouse epidermal growth factor (EGF) was covalently conjugated with the water-soluble polymer, poly(acrylic acid) (EGF-PAA), or with the water-insoluble polymer, surface-hydrolyzed poly(methyl methacrylate) (EGF-PMMA). Immobilized EGF (EGF-PMMA) stimulated DNA synthesis in Chinese hamster ovary cells...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1997-03, Vol.121 (3), p.514-520
Main Authors: Ito, Yoshihiro, Li, Jing-Song, Takahashi, Takashi, Imanishi, Yukio, Okabayashi, Yoshinori, Kido, Yoshiaki, Kasuga, Masato
Format: Article
Language:English
Subjects:
Acrylic Resins - chemistry
Animals
bioconjugate material
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
CHO Cells
Cricetinae
EGF
Enzyme Activation
Epidermal Growth Factor - chemistry
Epidermal Growth Factor - pharmacology
ErbB Receptors - metabolism
immobilization
juxtacrine
Methylmethacrylates - chemistry
Mice
Phosphorylation
tissue engineering
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Summary:Mouse epidermal growth factor (EGF) was covalently conjugated with the water-soluble polymer, poly(acrylic acid) (EGF-PAA), or with the water-insoluble polymer, surface-hydrolyzed poly(methyl methacrylate) (EGF-PMMA). Immobilized EGF (EGF-PMMA) stimulated DNA synthesis in Chinese hamster ovary cells overexpressing EGF receptors in amounts that were 5 to 10% of those of free EGF required for comparable effects. In addition, the maximal mitogenic effect of EGF-PMMA was greater than that of unconjugated EGF or EGF-PAA. EGF, EGF-PAA, and EGF-PMMA induced the autophosphorylation of EGF receptors and the stimulation of mitogen-activated protein kinase. However, whereas the onset of these effects was delayed with EGF-PMMA, they persisted for much longer than those of EGF and EGF-PAA. Unlike EGF and EGF-PAA, EGF-PMMA was not associated with cells after their removal from culture and did not induce receptor internalization. Culturing cells with PMMA-immobilized EGF thus represents a model system for studying “juxtacrine” stimulation of cells by membrane-bound growth factors.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a021616