Effects of altered cardiac membrane fluidity on β-adrenergic receptor signalling in rats with cirrhotic cardiomyopathy

Background/Aims: The relationship between cardiac plasma membrane physical properties and β-adrenergic receptor signalling function in cirrhotic cardiomyopathy remains unclear. We aimed to clarify this issue by examining the effect of altering membrane fluidity on β-adrenergic receptor signalling in...

Full description

Saved in:
Bibliographic Details
Published in:Journal of hepatology 1997-04, Vol.26 (4), p.904-912
Main Authors: Ma, Zenghua, Lee, Samuel S., Meddings, Jonathan B.
Format: Article
Language:English
Subjects:
Adenylyl cyclase: β-adrenoceptor
Animals
Biological and medical sciences
Cardiology. Vascular system
Cardiomyopathies - etiology
Cardiomyopathies - metabolism
Cardiomyopathies - physiopathology
Cell Membrane - metabolism
Cyclic AMP - biosynthesis
G-protein
Heart
Liver - metabolism
Liver Cirrhosis, Experimental - complications
Male
Medical sciences
Membrane Fluidity
Myocardial Contraction
Myocarditis. Cardiomyopathies
Myocardium - metabolism
Papillary Muscles - physiopathology
Rats
Rats, Sprague-Dawley
Receptors, Adrenergic, beta - physiology
Signal Transduction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background/Aims: The relationship between cardiac plasma membrane physical properties and β-adrenergic receptor signalling function in cirrhotic cardiomyopathy remains unclear. We aimed to clarify this issue by examining the effect of altering membrane fluidity on β-adrenergic receptor signalling in cirrhotic rats. Methods: Cirrhosis was induced by chronic bile duct ligation, while controls were sham-operated. Left ventricular papillary muscle contractility was measured in an organ bath containing 95% oxygen saturated Tyrode's buffer. Cardiac plasma membrane physical properties, represented by membrane fluidity, were evaluated by fluorescent depolarization, using 1,6-diphenyl-1,3,5,-hexatriene as a probe. Membrane cAMP levels were measured after stimulation at the β-adrenoceptor level by isoproterenol (10 −4 mol/l), the G-protein level with AIF −4 (10 mmol/l) and the adenylyl cyclase level with forskolin (100 μmol/l) before and after membrane fluidization with 2-(2-methoxyethoxy)ethyl 8-(cis-2-n-octylcyclopropyl)octanoate (A 2C). Results: Maximum papillary muscle contractile force under isoproterenol stimulation was 0.48±0.05 and 0.34±0.03 N/cm 2 in sham-operated and bile duct ligated rats, respectively ( p
ISSN:0168-8278
1600-0641
DOI:10.1016/S0168-8278(97)80259-0