Anti-β-crystallin Antibodies (Mouse) or Sera from Humans with Age-related Cataract are Cytotoxic for Lens Epithelial Cells in Culture

Circulating autoantibodies against lens antigens are prevalent in patients with age-related cataract (ARC), but their pathogenic significance is unknown. We hypothesized that these autoantibodies are cytotoxic for lens epithelial cells (LECs). To test this hypothesis, we incubated LECs with mouse po...

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Bibliographic Details
Published in:Experimental eye research 1997-02, Vol.64 (2), p.229-238
Main Authors: IBARAKI, NOBUHIRO, LIN, LI-REN, DANG, LOAN, REDDY, VENKAT N., SINGH, DHIRENDRA P., SUENO, TOSHIHARU, CHYLACK, LEO T., SHINOHARA, TOSHIMICHI
Format: Article
Language:English
Subjects:
age-related cataract
Animals
Antibodies - immunology
autoantibodies
Autoantibodies - blood
Autoantibodies - immunology
Binding Sites, Antibody
Biological and medical sciences
Cataract - blood
Cataract - immunology
Cell Differentiation
Cell Survival
Cells, Cultured
classical pathway
complement
Complement System Proteins - physiology
Crystallins - immunology
Cytotoxicity, Immunologic
Epithelium - immunology
Epithelium - pathology
human sera
Humans
Infant
Lens diseases
Lens, Crystalline - immunology
Lens, Crystalline - pathology
Medical sciences
Mice
Ophthalmology
TGF-β
β-crystallin
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Description
Summary:Circulating autoantibodies against lens antigens are prevalent in patients with age-related cataract (ARC), but their pathogenic significance is unknown. We hypothesized that these autoantibodies are cytotoxic for lens epithelial cells (LECs). To test this hypothesis, we incubated LECs with mouse polyclonal or monoclonal antibodies against β-crystallin (anti-β) in the presence or absence of guinea pig complement. We found that anti-β in the presence of the complement bound to and killed mouse LECs (MLECs) and human LECs (HLECs). Sera obtained from patients with ARC also were cytotoxic to both HLECs and MLECs in culture. Heat-inactivated human sera were not cytotoxic to LECs in the absence of the complement, but were cytotoxic to both HLECs and MLECs in the presence of additional complement. These results support the hypothesis that autoantibodies against lens antigens are cytotoxic to LECs, and that cell death may involve complement-mediated pathways.
ISSN:0014-4835
1096-0007
DOI:10.1006/exer.1996.0203