Inducible (type II) nitric oxide synthase in human placental villous tissue of normotensive, pre-eclamptic and intrauterine growth-restricted pregnancies

We have utilized two distinct monospecific antibodies against the type II (macrophage or inducible) nitric oxide synthase (NOS) isoform to localize the distribution of the enzyme within the human placenta in tissues from normotensive pregnancies and those complicated by pre-eclampsia and/or intraute...

Full description

Saved in:
Bibliographic Details
Published in:Placenta (Eastbourne) 1997-05, Vol.18 (4), p.261-268
Main Authors: Myatt, L., Eis, A.L.W., Brockman, D.E., Kossenjans, W., Greer, I., Lyall, F.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Diseases of mother, fetus and pregnancy
Female
Fetal Growth Retardation - enzymology
Fluorescent Antibody Technique
Gynecology. Andrology. Obstetrics
Humans
Immunoenzyme Techniques
Immunohistochemistry
Isoenzymes - analysis
Medical sciences
Nitric Oxide Synthase - analysis
Placenta - enzymology
Polymerase Chain Reaction
Pre-Eclampsia - enzymology
Pregnancy
Pregnancy. Fetus. Placenta
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have utilized two distinct monospecific antibodies against the type II (macrophage or inducible) nitric oxide synthase (NOS) isoform to localize the distribution of the enzyme within the human placenta in tissues from normotensive pregnancies and those complicated by pre-eclampsia and/or intrauterine growth restriction. Both antibodies immunolocalize to cells in the villous stroma on frozen sections of villous tissue. Colocalization studies with anti-CD14 or anti-CD45 antibodies that recognize cells of leucocyte or monocyte/macrophage lineage indicate that Hofbauer cells are expressing type II NOS. This is in contrast to expression of type III (endothelial) NOS which is seen in syncytiotrophoblast and in villous vascular endothelium. In some, but not all, normotensive and pathologic placental tissue, some type II NOS immunostaining could be seen in syncytiotrophoblast and vascular endothelium; however, no differences could bediscerned between groups of tissues. Expression of type II NOS by Hofbauer cells may indicate they are involved in surveillance against maternal immune insult or maternal pathogens whereby they secrete nitric oxide to exert a cytostatic/cytotoxic effect.
ISSN:0143-4004
1532-3102
DOI:10.1016/S0143-4004(97)80060-4