Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae

• Published in: FEBS letters 1997-05, Vol.408 (2), p.217-220
• Main Authors: Brody, Stuart, Oh, Changkyu, Hoja, Ursula, Schweizer, Eckhart
• Format: Article
• Language: English
• Subjects: ACP mutation; Acyl carrier protein; Acyl Carrier Protein - genetics; Acyl Carrier Protein - metabolism; Cloning, Molecular; FAS; fatty acid synthase; Fatty Acid Synthases - metabolism; Fatty Acids, Unsaturated - biosynthesis; Fatty Acids, Unsaturated - metabolism; Fungal Proteins - metabolism; Genes, Fungal; Lipoic acid; Meiosis; Mitochondria - enzymology; Mitochondria - metabolism; mitochondrial acyl carrier protein; Mitochondrial FAS; mtACP; Mutation; PCR; PKS; polyketide synthase; polymerase chain reaction; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Sequence Deletion; Thioctic Acid - analysis; Thioctic Acid - biosynthesis; Yeast
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(97)00428-6

The yeast gene, ACP1, encoding the mitochondrial acyl carrier protein, was deleted by gene replacement. The resulting acp1-deficient mutants had only 5–10% of the wild-type lipoic acid content remaining, and exhibited a respiratory-deficient phenotype. Upon meiosis, the lipoate deficiency co-segregated with the acp1 deletion. The role of ACP1 in long-chain fatty acid synthesis was studied in fas1 and fas2 null mutants completely lacking cytoplasmic fatty acid synthase. When grown on odd-chain (13:0 and 15:0) fatty acids, these cells showed less than 1% of C-16 and C-18 acids in their total lipids. Mitochondrial ACP is therefore suggested to be involved with the biosynthesis of octanoate, a precurser to lipoic acid.