Kinetic analysis and multiple component monitoring of effectors of adenylyl cyclase activity by quantitative fast-atom bombardment mass spectrometry

• Published in: Rapid communications in mass spectrometry 1997-01, Vol.11 (9), p.1060-1066
• Main Authors: Newton, Russell P., Bayliss, Mark A., van Geyschem, Jan, Harris, Frank M., Games, David E., Brenton, Gareth, Wilkins, Adam C. R., Diffley, Penny, Walton, Terence J.
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - analysis; Adenosine Triphosphate - metabolism; Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - metabolism; Animals; Brain - drug effects; Brain - enzymology; Cyclic AMP - analysis; Cyclic AMP - metabolism; Enzyme Inhibitors - pharmacology; Kinetics; Phosphodiesterase Inhibitors - pharmacology; Rats; Spectrometry, Mass, Fast Atom Bombardment
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/(SICI)1097-0231(19970615)11:9<1060::AID-RCM944>3.0.CO;2-3

The enzyme adenylyl cyclase catalyses the conversion of adenosine 5′‐triphosphate (ATP) to adenosine‐3′,5′‐cyclic monophosphate (cyclic AMP), and is an important pharmaceutical target. Quantitation of this enzyme's activity has been carried out by positive‐ion fast‐atom bombardment mass spectrometric analysis of the enzyme incubation mixture after the reaction has been terminated. The kinetic data obtained are in good agreement with those obtained by the conventional radiometric assay, and this mass spectrometry‐based assay offers the facility to monitor the turnover of several components of the incubation simultaneously. This is utilized to study the relative efficiencies of two ATP‐regenerating systems, three phosphodiesterase inhibitors and two modified substrates, and to monitor the uptake and conversion of two competing substrates, adenosine 5′ triphosphate and 2′‐deoxyadenosine‐5‐triphosphate, to cyclic AMP and to cyclic deoxyAMP, respectively. © 1997 John Wiley & Sons, Ltd.