Elevation of Ouabainlike Compound Levels With Hypertonic Sodium Chloride Load in Rat Plasma and Tissues

A major biologically active endogenous digitalis-like factor in the mammalian body may be an isomer of ouabain (ouabainlike compound, OLC). However, the exact role of OLC in sodium homeostasis is still unclear, and acute isotonic volume expansion does not enhance the secretion of OLC. We tested the...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 1997-07, Vol.30 (1), p.94-98
Main Authors: Yamada, Kaoru, Goto, Atsuo, Nagoshi, Hiroshi, Terano, Yoshitake, Omata, Masao
Format: Article
Language:English
Subjects:
Adrenal Glands - chemistry
Adrenal Glands - metabolism
Analysis of Variance
Animals
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Clinical manifestations. Epidemiology. Investigative techniques. Etiology
Homeostasis
Hypernatremia - metabolism
Hypertonic Solutions
Hypothalamus - chemistry
Hypothalamus - metabolism
Injections, Intraperitoneal
Isomerism
Isotonic Solutions
Male
Medical sciences
Ouabain - analysis
Ouabain - blood
Ouabain - metabolism
Pituitary Gland - chemistry
Pituitary Gland - metabolism
Potassium - blood
Radioimmunoassay
Rats
Rats, Wistar
Sodium - blood
Sodium Chloride - administration & dosage
Sodium Chloride - pharmacology
Software
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Summary:A major biologically active endogenous digitalis-like factor in the mammalian body may be an isomer of ouabain (ouabainlike compound, OLC). However, the exact role of OLC in sodium homeostasis is still unclear, and acute isotonic volume expansion does not enhance the secretion of OLC. We tested the hypothesis that OLC may be more important in the response to acute hypertonic NaCl load rather than isotonic volume expansion. We injected intraperitoneally 2 mL of 20% NaCl solution into male Wistar rats (n = 34) and measured OLC levels in plasma, hypothalamus, pituitary, and adrenal at baseline (n = 10) and 1, 2, and 4 hours (n = 8 for each). In response to hypertonic NaCl loading, plasma Na-K ratio was elevated at 2 and 4 hours (P < .01). OLC levels in pituitary increased (P < .01) at 1 hour. Thereafter, plasma OLC levels increased at 2 and 4 hours (P < .05; basal, 75 +/- 11 pmol/L [+/- SEM]; 1 hour, 55 +/- 11; 2 hours, 130 +/- 24; 4 hours, 156 +/- 20). Concomitantly, OLC levels in adrenal increased at 2 and 4 hours (P < .01; basal, 1.7 +/- 0.2 pmol/g; 1 hour, 4.5 +/- 0.9; 2 hours, 5.0 +/- 0.7; 4 hours, 6.8 +/- 2.2). A significant correlation was observed between OLC levels in plasma and adrenal (P
ISSN:0194-911X
1524-4563
DOI:10.1161/01.HYP.30.1.94