Glutamine transport in human and rat placenta

• Published in: Placenta (Eastbourne) 1997-07, Vol.18 (5), p.379-386
• Main Authors: Novak, D.A., Beveridge, M.J.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biological Transport; Female; Fundamental and applied biological sciences. Psychology; Glutamine - metabolism; Humans; Maternal-Fetal Exchange; Mother. Fetoplacental unit. Mammary gland. Milk; Placenta - metabolism; Pregnancy; Pregnancy. Parturition. Lactation; Rats; Rats, Sprague-Dawley; Sodium - metabolism; Vertebrates: reproduction
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/S0143-4004(97)80037-9

Glutamine plays an important role in fetal nutrition. This study explored the transport of [ 3H]glutamine into apical and basal predominant membrane vesicles derived from rat and human placenta. Na +-dependent glutamine transport was present in both apical and basal predominant vesicles derived from 20- and, to a lesser degree, 14-day gestation rat placenta. Amino-acid transport systems A, ASC-like, B o,+ (in apical membrane vesicles) and, perhaps, y +L were involved in Na +-dependent glutamine transport. Na +-dependent glutamine uptake into human placental microvillus and basolateral membrane vesicles also occurred via several distinct transport activities. Glutamine transport via system N was not detected in either rat or human placental preparations. Na +-dependent glutamine transport in the rat was more pronounced in basal as compared to apical membrane vesicles. Conversely, in the human preparations, activity was significantly higher in microvillus as compared to basolateral membrane vesicles. It is concluded that Na +-dependent glutamine transport occurs through a variety of transport agencies in both the rat and human placenta. Transport varies with ontogeny and between species.